Oxide nanowire microfluidic devices for capturing single-stranded dnas

Marina MUSA, Takao YASUI, Zetao ZHU, Kazuki Nagashima, Miki ONO, Quanli LIU, Hiromi TAKAHASHI, Taisuke SHIMADA, Akihide ARIMA, Takeshi YANAGIDA, Yoshinobu BABA

Research output: Contribution to journalArticlepeer-review

2 Citations (Scopus)

Abstract

Since DNA analysis is the fundamental process for most applications in biomedical fields, capturing DNAs with high efficiency is important. Here, we used several oxide nanowire microfluidic devices to capture CpG-rich single-stranded DNAs (ssDNAs) in different pH solutions. All the oxide nanowires exhibited the highest capture efficiency around pH 7 with good capture efficiency shown by each metal oxide; ZnO/ZnO core/shell NWs (71.6%), ZnO/Al2O3 core/shell NWs (86.3%) and ZnO/SiO2 core/shell NWs (86.7%). ZnO/Al2O3 core/shell NWs showed the best performance for capturing ssDNAs under varying pH, which suggests its suitability for application in diverse biological fluids. The capturing efficiencies were attributed to the interactions from phosphate backbones and nucleobases of ssDNAs to each nanowire surface. This finding provides a useful platform for highly efficient capture of the target ssDNAs, and these results can be extended for future studies of cancer-related genes in complex biological fluids.

Original languageEnglish
Pages (from-to)1139-1145
Number of pages7
Journalanalytical sciences
Volume37
Issue number8
DOIs
Publication statusPublished - 2021

All Science Journal Classification (ASJC) codes

  • Analytical Chemistry

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