Oxygen-binding Protein Fiber and Microgel: Supramolecular Myoglobin-Poly(acrylate) Conjugates

Toshikazu Ono, Yasushi Hisaoka, Akira Onoda, Koji Oohora, Takashi Hayashi

Research output: Contribution to journalArticlepeer-review

7 Citations (Scopus)

Abstract

A supramolecular conjugate of myoglobin (Mb) and water-soluble poly(acrylate), (PA5k and PA25k, where 5k and 25k represent the molecular weight of the polymers, respectively), is constructed on the basis of a noncovalent heme-heme pocket interaction. The modified heme with an amino group linked to the terminus of one of the heme-propionates is coupled to the side-chain carboxyl groups of poly(acrylate) activated by N-hydroxysuccinimide. The ratios of the heme-modified monomer unit and the unmodified monomer unit (m:n) in the polymer chains of Heme-PA5k and Heme-PA25k were determined to be 4.5:95.5 and 3.1:96.9, respectively. Subsequent addition of apoMb to the conjugated polymers provides Mb-connected fibrous nanostructures confirmed by atomic force microscopy. A mixture of the heme-modified polymer and dimeric apomyoglobin as a cross-linker forms a microgel in which the reconstituted myoglobin retains its native exogenous ligand binding activity.

Original languageEnglish
Pages (from-to)1036-1042
Number of pages7
JournalChemistry - An Asian Journal
Volume11
Issue number7
DOIs
Publication statusPublished - Apr 5 2016

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

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