Oxygen-binding Protein Fiber and Microgel: Supramolecular Myoglobin-Poly(acrylate) Conjugates

Toshikazu Ono, Yasushi Hisaoka, Akira Onoda, Koji Oohora, Takashi Hayashi

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

A supramolecular conjugate of myoglobin (Mb) and water-soluble poly(acrylate), (PA5k and PA25k, where 5k and 25k represent the molecular weight of the polymers, respectively), is constructed on the basis of a noncovalent heme-heme pocket interaction. The modified heme with an amino group linked to the terminus of one of the heme-propionates is coupled to the side-chain carboxyl groups of poly(acrylate) activated by N-hydroxysuccinimide. The ratios of the heme-modified monomer unit and the unmodified monomer unit (m:n) in the polymer chains of Heme-PA5k and Heme-PA25k were determined to be 4.5:95.5 and 3.1:96.9, respectively. Subsequent addition of apoMb to the conjugated polymers provides Mb-connected fibrous nanostructures confirmed by atomic force microscopy. A mixture of the heme-modified polymer and dimeric apomyoglobin as a cross-linker forms a microgel in which the reconstituted myoglobin retains its native exogenous ligand binding activity.

Original languageEnglish
Pages (from-to)1036-1042
Number of pages7
JournalChemistry - An Asian Journal
Volume11
Issue number7
DOIs
Publication statusPublished - Apr 5 2016

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Myoglobin
Heme
Carrier Proteins
Oxygen
Fibers
Polymers
Monomers
acrylic acid
Nanostructures
Atomic Force Microscopy
Conjugated polymers
Propionates
Atomic force microscopy
Molecular Weight
Molecular weight
Ligands
Water

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Organic Chemistry

Cite this

Oxygen-binding Protein Fiber and Microgel : Supramolecular Myoglobin-Poly(acrylate) Conjugates. / Ono, Toshikazu; Hisaoka, Yasushi; Onoda, Akira; Oohora, Koji; Hayashi, Takashi.

In: Chemistry - An Asian Journal, Vol. 11, No. 7, 05.04.2016, p. 1036-1042.

Research output: Contribution to journalArticle

Ono, Toshikazu ; Hisaoka, Yasushi ; Onoda, Akira ; Oohora, Koji ; Hayashi, Takashi. / Oxygen-binding Protein Fiber and Microgel : Supramolecular Myoglobin-Poly(acrylate) Conjugates. In: Chemistry - An Asian Journal. 2016 ; Vol. 11, No. 7. pp. 1036-1042.
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