Oxygen metabolism analysis of a vascularized spheroid using a scanning electrochemical microscopy

Yuji Nashimoto, Rei Mukomoto, Takato Terai, Kosuke Ino, Koichi Nishiyama, Ryuji Yokokawa, Takashi Miura, Hitoshi Shiku

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We investigated the effect of vascularization on oxygen metabolism in a fibroblast spheroid. By the scanning of a microelectrode, the oxygen reduction current was monitored, which allowed us to analyze the oxygen metabolism quantitatively. The oxygen metabolism of the spheroids were determined by the spheroid radius (volume) and did not depend on whether the spheroid was vascularized or not, implying the identical activity of oxygen metabolism of the fibroblast and endothelial cells. Our system is useful for the metabolic evaluation of a three-dimensional culture model.

Original languageEnglish
Title of host publicationMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages520-521
Number of pages2
ISBN (Electronic)9781733419017
Publication statusPublished - 2020
Event24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020 - Virtual, Online
Duration: Oct 4 2020Oct 9 2020

Publication series

NameMicroTAS 2020 - 24th International Conference on Miniaturized Systems for Chemistry and Life Sciences

Conference

Conference24th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2020
CityVirtual, Online
Period10/4/2010/9/20

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering
  • Chemistry(all)
  • Control and Systems Engineering

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