Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG

Shuntaro Tsukamoto; Shuya Yamashita; Yoon Hee Kim; Motofumi Kumazoe; Yuhui Huang; Koji Yamada; Hirofumi Tachibana

doi:10.1016/j.febslet.2012.07.065

Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG

Shuntaro Tsukamoto, Shuya Yamashita, Yoon Hee Kim, Motofumi Kumazoe, Yuhui Huang, Koji Yamada, Hirofumi Tachibana

Food Science and Biotechnology

Research output: Contribution to journal › Article › peer-review

12 Citations (Scopus)

Abstract

(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O₂ levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O₂ levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O₂. In summary, 67LR expression is highly sensitive to O₂ partial pressure, and the activity of EGCG can be regulated in cancer cells by O₂ partial pressure.

Original language	English
Pages (from-to)	3441-3447
Number of pages	7
Journal	FEBS Letters
Volume	586
Issue number	19
DOIs	https://doi.org/10.1016/j.febslet.2012.07.065
Publication status	Published - Sep 21 2012

All Science Journal Classification (ASJC) codes

Biophysics
Structural Biology
Biochemistry
Molecular Biology
Genetics
Cell Biology

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Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG. / Tsukamoto, Shuntaro; Yamashita, Shuya; Kim, Yoon Hee; Kumazoe, Motofumi; Huang, Yuhui; Yamada, Koji; Tachibana, Hirofumi.

In: FEBS Letters, Vol. 586, No. 19, 21.09.2012, p. 3441-3447.

Research output: Contribution to journal › Article › peer-review

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title = "Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG",

abstract = "(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O2 levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O2. In summary, 67LR expression is highly sensitive to O2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O2 partial pressure.",

author = "Shuntaro Tsukamoto and Shuya Yamashita and Kim, {Yoon Hee} and Motofumi Kumazoe and Yuhui Huang and Koji Yamada and Hirofumi Tachibana",

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AU - Tsukamoto, Shuntaro

AU - Yamashita, Shuya

AU - Kim, Yoon Hee

AU - Kumazoe, Motofumi

AU - Huang, Yuhui

AU - Yamada, Koji

AU - Tachibana, Hirofumi

PY - 2012/9/21

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AB - (-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O2 levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O2. In summary, 67LR expression is highly sensitive to O2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O2 partial pressure.

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