TY - JOUR
T1 - Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG
AU - Tsukamoto, Shuntaro
AU - Yamashita, Shuya
AU - Kim, Yoon Hee
AU - Kumazoe, Motofumi
AU - Huang, Yuhui
AU - Yamada, Koji
AU - Tachibana, Hirofumi
N1 - Funding Information:
This work was supported in part by a Grants-in-Aid for Scientific Research (S) (grant number 22228002; to H.T.) and Research Fellowships from the Japan Society for the Promotion of Science for Young (DC2; to S.T.).
Copyright:
Copyright 2013 Elsevier B.V., All rights reserved.
PY - 2012/9/21
Y1 - 2012/9/21
N2 - (-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O2 levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O2. In summary, 67LR expression is highly sensitive to O2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O2 partial pressure.
AB - (-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O2 levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O2. In summary, 67LR expression is highly sensitive to O2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O2 partial pressure.
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U2 - 10.1016/j.febslet.2012.07.065
DO - 10.1016/j.febslet.2012.07.065
M3 - Article
C2 - 22884420
AN - SCOPUS:84866613751
VL - 586
SP - 3441
EP - 3447
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 19
ER -
