Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG

Shuntaro Tsukamoto, Shuya Yamashita, Yoon Hee Kim, Motofumi Kumazoe, Yuhui Huang, Koji Yamada, Hirofumi Tachibana

Research output: Contribution to journalArticlepeer-review

12 Citations (Scopus)

Abstract

(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O2 levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O2. In summary, 67LR expression is highly sensitive to O2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O2 partial pressure.

Original languageEnglish
Pages (from-to)3441-3447
Number of pages7
JournalFEBS Letters
Volume586
Issue number19
DOIs
Publication statusPublished - Sep 21 2012

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Structural Biology
  • Biochemistry
  • Molecular Biology
  • Genetics
  • Cell Biology

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