Abstract
(-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O2 levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O2. In summary, 67LR expression is highly sensitive to O2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O2 partial pressure.
| Original language | English |
|---|---|
| Pages (from-to) | 3441-3447 |
| Number of pages | 7 |
| Journal | FEBS Letters |
| Volume | 586 |
| Issue number | 19 |
| DOIs | |
| Publication status | Published - Sep 21 2012 |
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All Science Journal Classification (ASJC) codes
- Biophysics
- Structural Biology
- Biochemistry
- Molecular Biology
- Genetics
- Cell Biology
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Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG. / Tsukamoto, Shuntaro; Yamashita, Shuya; Kim, Yoon Hee; Kumazoe, Motofumi; Huang, Yuhui; Yamada, Koji; Tachibana, Hirofumi.
In: FEBS Letters, Vol. 586, No. 19, 21.09.2012, p. 3441-3447.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Oxygen partial pressure modulates 67-kDa laminin receptor expression, leading to altered activity of the green tea polyphenol, EGCG
AU - Tsukamoto, Shuntaro
AU - Yamashita, Shuya
AU - Kim, Yoon Hee
AU - Kumazoe, Motofumi
AU - Huang, Yuhui
AU - Yamada, Koji
AU - Tachibana, Hirofumi
PY - 2012/9/21
Y1 - 2012/9/21
N2 - (-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O2 levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O2. In summary, 67LR expression is highly sensitive to O2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O2 partial pressure.
AB - (-)-Epigallocatechin-3-O-gallate (EGCG) exhibits anti-tumor activity mediated via the 67-kDa laminin receptor (67LR). In this study, we found that 67LR protein levels are reduced by exposure to low O2 levels (5%), without affecting the expression of HIF-1α. We also found that EGCG-induced anti-cancer activity is abrogated under low O2 levels (5%) in various cancer cells. Notably, treatment with the proteasome inhibitor, prevented down-regulation of 67LR and restored sensitivity to EGCG under 5% O2. In summary, 67LR expression is highly sensitive to O2 partial pressure, and the activity of EGCG can be regulated in cancer cells by O2 partial pressure.
UR - http://www.scopus.com/inward/record.url?scp=84866613751&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84866613751&partnerID=8YFLogxK
U2 - 10.1016/j.febslet.2012.07.065
DO - 10.1016/j.febslet.2012.07.065
M3 - Article
C2 - 22884420
AN - SCOPUS:84866613751
VL - 586
SP - 3441
EP - 3447
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 19
ER -