Oxysterol regulation of estrogen receptor α-mediated gene expression in a transcriptional activation assay system using HeLa cells

Hiroyoshi Sato, Satoko Nishida, Hiroko Tomoyori, Masao Sato, Ikuo Ikeda, Katsumi Imaizumi

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

In order to test the estrogenic activity of sterol oxidation products from cholesterol and phytosterols, an estrogen-dependent gene expression assay was performed in estrogen receptor α-stably transformed HeLa cells. The ranking of the estrogenic potency of these compounds was different: 17β-estradiol ≫ genistein ≫ β-epoxycholesterol = daidzein = cholestanetriol = 22(R)-hydroxycholesterol = 20(S)-hydroxycholesterol = sitostanetriol > campestanetriol = β-epoxysitosterol = 7β-hydroxycholesterol. These compounds were not estrogenic in estrogen receptor-negative HeLa cells.

Original languageEnglish
Pages (from-to)1790-1793
Number of pages4
JournalBioscience, Biotechnology and Biochemistry
Volume68
Issue number8
DOIs
Publication statusPublished - Aug 1 2004

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Analytical Chemistry
  • Biochemistry
  • Applied Microbiology and Biotechnology
  • Molecular Biology
  • Organic Chemistry

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