P32/gC1qR is indispensable for fetal development and mitochondrial translation

Importance of its RNA-binding ability

Mikako Yagi, Takeshi Uchiumi, Shinya Takazaki, Bungo Okuno, Masatoshi Nomura, Shin Ichi Yoshida, Tomotake Kanki, Dongchon Kang

Research output: Contribution to journalArticle

55 Citations (Scopus)

Abstract

p32 is an evolutionarily conserved and ubiquitously expressed multifunctional protein. Although p32 exists at diverse intra and extracellular sites, it is predominantly localized to the mitochondrial matrix near the nucleoid associated with mitochondrial transcription factor A. Nonetheless, its function in the matrix is poorly understood. Here, we determined p32 function via generation of p32-knockout mice. p32-deficient mice exhibited midgestation lethality associated with a severe developmental defect of the embryo. Primary embryonic fibroblasts isolated from p32-knockout embryos showed severe dysfunction of the mitochondrial respiratory chain, because of severely impaired mitochondrial protein synthesis. Recombinant p32 binds RNA, not DNA, and endogenous p32 interacts with all mitochondrial messenger RNA species in vivo. The RNA-binding ability of p32 is well correlated with the mitochondrial translation. Coimmunoprecipitation revealed the close association of p32 with the mitoribosome. We propose that p32 is required for functional mitoribosome formation to synthesize proteins within mitochondria.

Original languageEnglish
Pages (from-to)9717-9737
Number of pages21
JournalNucleic Acids Research
Volume40
Issue number19
DOIs
Publication statusPublished - Oct 1 2012

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Fetal Development
Embryonic Structures
RNA
Mitochondrial Proteins
Electron Transport
Knockout Mice
Mitochondria
Proteins
Fibroblasts
DNA
Mitochondrial Ribosomes
mitochondrial messenger RNA
mitochondrial transcription factor A

All Science Journal Classification (ASJC) codes

  • Genetics

Cite this

P32/gC1qR is indispensable for fetal development and mitochondrial translation : Importance of its RNA-binding ability. / Yagi, Mikako; Uchiumi, Takeshi; Takazaki, Shinya; Okuno, Bungo; Nomura, Masatoshi; Yoshida, Shin Ichi; Kanki, Tomotake; Kang, Dongchon.

In: Nucleic Acids Research, Vol. 40, No. 19, 01.10.2012, p. 9717-9737.

Research output: Contribution to journalArticle

Yagi, Mikako ; Uchiumi, Takeshi ; Takazaki, Shinya ; Okuno, Bungo ; Nomura, Masatoshi ; Yoshida, Shin Ichi ; Kanki, Tomotake ; Kang, Dongchon. / P32/gC1qR is indispensable for fetal development and mitochondrial translation : Importance of its RNA-binding ability. In: Nucleic Acids Research. 2012 ; Vol. 40, No. 19. pp. 9717-9737.
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