TY - JOUR
T1 - p37 Is a p97 Adaptor Required for Golgi and ER Biogenesis in Interphase and at the End of Mitosis
AU - Uchiyama, Keiji
AU - Totsukawa, Go
AU - Puhka, Maija
AU - Kaneko, Yayoi
AU - Jokitalo, Eija
AU - Dreveny, Ingrid
AU - Beuron, Fabienne
AU - Zhang, Xiaodong
AU - Freemont, Paul
AU - Kondo, Hisao
PY - 2006/12
Y1 - 2006/12
N2 - We previously reported that p97/p47-assisted membrane fusion is important for the reassembly of organelles at the end of mitosis, but not for their maintenance during interphase. We have now identified a p97 adaptor protein, p37, which forms a complex with p97 in the cytosol and localizes to the Golgi and ER. siRNA experiments revealed that p37 is required for Golgi and ER biogenesis. Injection of anti-p37 antibodies into cells at different cell cycle stages showed that p37 plays an important role in both Golgi and ER maintenance during interphase as well as in their reassembly at the end of mitosis. In an in vitro Golgi reassembly assay, the p97/p37 complex has membrane fusion activity. In contrast to the p97/p47 pathway, this pathway requires p115-GM130 tethering and SNARE GS15, but not syntaxin5. Interestingly, although VCIP135 is also required, its deubiquitinating activity is unnecessary for p97/p37-mediated activities.
AB - We previously reported that p97/p47-assisted membrane fusion is important for the reassembly of organelles at the end of mitosis, but not for their maintenance during interphase. We have now identified a p97 adaptor protein, p37, which forms a complex with p97 in the cytosol and localizes to the Golgi and ER. siRNA experiments revealed that p37 is required for Golgi and ER biogenesis. Injection of anti-p37 antibodies into cells at different cell cycle stages showed that p37 plays an important role in both Golgi and ER maintenance during interphase as well as in their reassembly at the end of mitosis. In an in vitro Golgi reassembly assay, the p97/p37 complex has membrane fusion activity. In contrast to the p97/p47 pathway, this pathway requires p115-GM130 tethering and SNARE GS15, but not syntaxin5. Interestingly, although VCIP135 is also required, its deubiquitinating activity is unnecessary for p97/p37-mediated activities.
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U2 - 10.1016/j.devcel.2006.10.016
DO - 10.1016/j.devcel.2006.10.016
M3 - Article
C2 - 17141156
AN - SCOPUS:33751343672
VL - 11
SP - 803
EP - 816
JO - Developmental Cell
JF - Developmental Cell
SN - 1534-5807
IS - 6
ER -