TY - JOUR
T1 - p40phox as an alternative organizer to p47phox in Nox2 activation
T2 - A new mechanism involving an interaction with p22phox
AU - Tamura, Minoru
AU - Shiozaki, Iichiro
AU - Ono, Shohei
AU - Miyano, Kei
AU - Kunihiro, Sachio
AU - Sasaki, Takayuki
PY - 2007/9/18
Y1 - 2007/9/18
N2 - p40phox activated phagocyte NADPH oxidase without p47phox in a cell-free system consisting of p67phox, Rac and cytochrome b558 relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70% of that by p47phox. Addition of p47phox slightly increased the activation, but not additively. p40phox improved the efficiency of p67phox in the activation. The C-terminus-truncated p67phox, p40phox(D289A), p40phox(R58A), or p40phox(W207R) showed an impaired activation. A peptide corresponding to the p22phox Pro-rich region suppressed the activation, and far-western blotting revealed its interaction with p40phox SH3 domain. Thus, p40phox can substitute for p47phox in the activation, interacting with p22phox and p67phox through their specific regions.
AB - p40phox activated phagocyte NADPH oxidase without p47phox in a cell-free system consisting of p67phox, Rac and cytochrome b558 relipidated with phosphatidylinositol 3-phosphate. The activation reached to 70% of that by p47phox. Addition of p47phox slightly increased the activation, but not additively. p40phox improved the efficiency of p67phox in the activation. The C-terminus-truncated p67phox, p40phox(D289A), p40phox(R58A), or p40phox(W207R) showed an impaired activation. A peptide corresponding to the p22phox Pro-rich region suppressed the activation, and far-western blotting revealed its interaction with p40phox SH3 domain. Thus, p40phox can substitute for p47phox in the activation, interacting with p22phox and p67phox through their specific regions.
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U2 - 10.1016/j.febslet.2007.08.040
DO - 10.1016/j.febslet.2007.08.040
M3 - Article
C2 - 17803994
AN - SCOPUS:34548388778
VL - 581
SP - 4533
EP - 4538
JO - FEBS Letters
JF - FEBS Letters
SN - 0014-5793
IS - 23
ER -