TY - JOUR
T1 - p53 gene mutations in soft-tissue sarcomas - Correlations with p53 immunohistochemistry and DNA ploidy
AU - Schneider-Stock, Regine
AU - Radig, Kathrin
AU - Oda, Yoshinao
AU - Mellin, Walter
AU - Rys, Janusz
AU - Niezabitowski, Aleksander
AU - Roessner, Albert
N1 - Funding Information:
Acknowledgements The authors are grateful to Prof. Jtrg T. Epplen, Bochum, Germany, his critical comments and helpful discussion: We thank Mrs. Hiltraud Scharfenort, Mrs. lngrid Roeseler, Mrs. Kathrin L~ther and Ms. Antje Leddin for excellent technical assistance and Mr. Bernd Wtisthoff for manuscript preparation. The work was supported by Deutsche Krebshilfe (10-1074-Schnl) and the Rudolf-Bartling-Foundation, Hanover, Germany. Yoshinao Oda was a recipient of an Alexander-yon-HumboldtF ellowship.
PY - 1997
Y1 - 1997
N2 - The significance of p53 mutations in a group of 67 soft-tissue tumors was examined using single-strand conformation polymorphism and direct sequencing analysis. Molecular findings were correlated with immunohistochemical detection of the p53 protein and DNA ploidy status. Mutations of the p53 gene were detected in 13 (19.5%) out of 67 cases of soft-tissue tumors. Only three were localized outside the conservative regions of the p53 gene. Six mutations were described for the first time in these tumors. Most of the mutations were point mutations in exons 5-8 and, in one case, a deletion at the 3'-splice site of exon 5 could be demonstrated. There was no significant correlation between the occurrence of p53 mutations and the histological grade, although a high number of mutations were defined in poorly differentiated tumors (grade 3). Molecular finding of a p53 gene mutation and immunohistochemical detection of p53 expression did not correlate, which may be due to the high percentage of nonsense mutations in our study (50%). We confirm that only DNA sequencing allows a unique identification and differentiation of mutations in the p53 gene. Other factors may be responsible for the detection of p53 protein in many cases. Histological grade correlated with aneuploidy. The frequency of mutations observed was in accordance with values quoted in the literature. Generally, p53 mutations and p53 overexpression are more likely to represent a late event in the oncogenesis of soft-tissue tumors.
AB - The significance of p53 mutations in a group of 67 soft-tissue tumors was examined using single-strand conformation polymorphism and direct sequencing analysis. Molecular findings were correlated with immunohistochemical detection of the p53 protein and DNA ploidy status. Mutations of the p53 gene were detected in 13 (19.5%) out of 67 cases of soft-tissue tumors. Only three were localized outside the conservative regions of the p53 gene. Six mutations were described for the first time in these tumors. Most of the mutations were point mutations in exons 5-8 and, in one case, a deletion at the 3'-splice site of exon 5 could be demonstrated. There was no significant correlation between the occurrence of p53 mutations and the histological grade, although a high number of mutations were defined in poorly differentiated tumors (grade 3). Molecular finding of a p53 gene mutation and immunohistochemical detection of p53 expression did not correlate, which may be due to the high percentage of nonsense mutations in our study (50%). We confirm that only DNA sequencing allows a unique identification and differentiation of mutations in the p53 gene. Other factors may be responsible for the detection of p53 protein in many cases. Histological grade correlated with aneuploidy. The frequency of mutations observed was in accordance with values quoted in the literature. Generally, p53 mutations and p53 overexpression are more likely to represent a late event in the oncogenesis of soft-tissue tumors.
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U2 - 10.1007/s004320050049
DO - 10.1007/s004320050049
M3 - Article
C2 - 9177493
AN - SCOPUS:0030994960
VL - 123
SP - 211
EP - 218
JO - Journal of Cancer Research and Clinical Oncology
JF - Journal of Cancer Research and Clinical Oncology
SN - 0171-5216
IS - 4
ER -