p73 interacts with c-Myc to regulate Y-box-binding protein-1 expression

Hidetaka Uramoto, Hiroto Izumi, Tomoko Ise, Mitsuhiro Tada, Takeshi Uchiumi, Michihiko Kuwano, Kosei Yasumoto, Keiko Funa, Kimitoshi Kohno

Research output: Contribution to journalArticle

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Abstract

YB-1 is a member of the cold shock domain family of proteins that is important for signaling DNA damage and cell proliferation. YB-1 is induced by DNA damage and can also recognize cisplatin-modified DNA. In this study we observed a 6-fold increase in the steady-state level of YB-1 mRNA in response to cisplatin exposure in cells of the human cancer cell line KB. We present evidence from cotransfection experiments for a critical role of c-Myc and p73 in the transactivation of the YB-1 promoter. p73 transactivated the YB-1 promoter in experiments with Saos-2 cells, which express c-Myc, but not with HO15.19 cells, which lack c-Myc. In turn, c-Myc transactivated an intact YB-1 promoter but not a YB-1 promoter with a mutant E-box, indicating that the E-box is necessary for the response of the promoter to cisplatin. We also found that p73 interacts with c-Myc in vitro and in vivo. Using deletion mutants we showed that the DNA-binding domain of p73 and the C-terminal region of c-Myc are required for the interaction. Furthermore, p73 stimulated the interaction of Max with c-Myc and promoted binding of the c-Myc-Max complex to its target DNA. Our data suggest that p73 stimulates the transcription of the YB-1 promoter by enhancing recruitment of the c-Myc-Max complex to the E-box.

Original languageEnglish
Pages (from-to)31694-31702
Number of pages9
JournalJournal of Biological Chemistry
Volume277
Issue number35
DOIs
Publication statusPublished - Aug 30 2002

Fingerprint

Y-Box-Binding Protein 1
Cisplatin
DNA Damage
DNA
Transcriptional Activation
Cells
Shock
Cell Proliferation
Cell proliferation
Cell Line
Transcription
Messenger RNA
Experiments
Neoplasms

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Uramoto, H., Izumi, H., Ise, T., Tada, M., Uchiumi, T., Kuwano, M., ... Kohno, K. (2002). p73 interacts with c-Myc to regulate Y-box-binding protein-1 expression. Journal of Biological Chemistry, 277(35), 31694-31702. https://doi.org/10.1074/jbc.M200266200

p73 interacts with c-Myc to regulate Y-box-binding protein-1 expression. / Uramoto, Hidetaka; Izumi, Hiroto; Ise, Tomoko; Tada, Mitsuhiro; Uchiumi, Takeshi; Kuwano, Michihiko; Yasumoto, Kosei; Funa, Keiko; Kohno, Kimitoshi.

In: Journal of Biological Chemistry, Vol. 277, No. 35, 30.08.2002, p. 31694-31702.

Research output: Contribution to journalArticle

Uramoto, H, Izumi, H, Ise, T, Tada, M, Uchiumi, T, Kuwano, M, Yasumoto, K, Funa, K & Kohno, K 2002, 'p73 interacts with c-Myc to regulate Y-box-binding protein-1 expression', Journal of Biological Chemistry, vol. 277, no. 35, pp. 31694-31702. https://doi.org/10.1074/jbc.M200266200
Uramoto, Hidetaka ; Izumi, Hiroto ; Ise, Tomoko ; Tada, Mitsuhiro ; Uchiumi, Takeshi ; Kuwano, Michihiko ; Yasumoto, Kosei ; Funa, Keiko ; Kohno, Kimitoshi. / p73 interacts with c-Myc to regulate Y-box-binding protein-1 expression. In: Journal of Biological Chemistry. 2002 ; Vol. 277, No. 35. pp. 31694-31702.
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