PACAP stimulates the release of interleukin-6 in cultured rat Müller cells

T. Seki, Y. Hinohara, C. Taki, M. Nakatani, M. Ozawa, S. Nishimura, Atsushi Takaki, H. Itho, F. Takenoya, S. Shioda

Research output: Contribution to journalArticle

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Abstract

We have investigated the in vivo effect of PACAP on rat Müller cells that are the predominant glial element in the retina. Müller cells were treated with PACAP38, either alone or in the presence of the PACAP-selective antagonist, PACAP6-38. Cellular proliferation was determined by measuring the incorporation of bromodeoxyuridine, while interleukin-6 (IL-6) levels in the culture medium were examined using a B9 cell bioassay. In cultured rat Müller cells, the expression of PACAP receptor (PAC1-R) was assessed with immunohistochemistry using a PAC1-R-specific antiserum. PACAP stimulated IL-6 production in Müller cells at a concentration as low as 10-12 M, which was not sufficient to induce cell proliferation. This elevation of IL-6 production was significantly inhibited by PACAP6-38. These data suggest that Müller cells are one of the target cells for PACAP, stimulating the release of IL-6, and providing a mechanism whereby PACAP exerts a significant neuroprotective effect in the retina.

Original languageEnglish
Pages (from-to)535-539
Number of pages5
JournalAnnals of the New York Academy of Sciences
Volume1070
DOIs
Publication statusPublished - Jan 1 2006

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Pituitary Adenylate Cyclase-Activating Polypeptide
Rats
Interleukin-6
Retina
Pituitary Adenylate Cyclase-Activating Polypeptide Receptors
Cell Proliferation
Bioassay
Cell proliferation
Neuroprotective Agents
Bromodeoxyuridine
Neuroglia
Biological Assay
Culture Media
Rat
Cells
Immune Sera
Immunohistochemistry

All Science Journal Classification (ASJC) codes

  • Neuroscience(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • History and Philosophy of Science

Cite this

Seki, T., Hinohara, Y., Taki, C., Nakatani, M., Ozawa, M., Nishimura, S., ... Shioda, S. (2006). PACAP stimulates the release of interleukin-6 in cultured rat Müller cells. Annals of the New York Academy of Sciences, 1070, 535-539. https://doi.org/10.1196/annals.1317.043

PACAP stimulates the release of interleukin-6 in cultured rat Müller cells. / Seki, T.; Hinohara, Y.; Taki, C.; Nakatani, M.; Ozawa, M.; Nishimura, S.; Takaki, Atsushi; Itho, H.; Takenoya, F.; Shioda, S.

In: Annals of the New York Academy of Sciences, Vol. 1070, 01.01.2006, p. 535-539.

Research output: Contribution to journalArticle

Seki, T, Hinohara, Y, Taki, C, Nakatani, M, Ozawa, M, Nishimura, S, Takaki, A, Itho, H, Takenoya, F & Shioda, S 2006, 'PACAP stimulates the release of interleukin-6 in cultured rat Müller cells', Annals of the New York Academy of Sciences, vol. 1070, pp. 535-539. https://doi.org/10.1196/annals.1317.043
Seki, T. ; Hinohara, Y. ; Taki, C. ; Nakatani, M. ; Ozawa, M. ; Nishimura, S. ; Takaki, Atsushi ; Itho, H. ; Takenoya, F. ; Shioda, S. / PACAP stimulates the release of interleukin-6 in cultured rat Müller cells. In: Annals of the New York Academy of Sciences. 2006 ; Vol. 1070. pp. 535-539.
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