TY - JOUR
T1 - PACAP stimulates the release of interleukin-6 in cultured rat Müller cells
AU - Seki, T.
AU - Hinohara, Y.
AU - Taki, C.
AU - Nakatani, M.
AU - Ozawa, M.
AU - Nishimura, S.
AU - Takaki, A.
AU - Itho, H.
AU - Takenoya, F.
AU - Shioda, S.
N1 - Copyright:
Copyright 2018 Elsevier B.V., All rights reserved.
PY - 2006/7
Y1 - 2006/7
N2 - We have investigated the in vivo effect of PACAP on rat Müller cells that are the predominant glial element in the retina. Müller cells were treated with PACAP38, either alone or in the presence of the PACAP-selective antagonist, PACAP6-38. Cellular proliferation was determined by measuring the incorporation of bromodeoxyuridine, while interleukin-6 (IL-6) levels in the culture medium were examined using a B9 cell bioassay. In cultured rat Müller cells, the expression of PACAP receptor (PAC1-R) was assessed with immunohistochemistry using a PAC1-R-specific antiserum. PACAP stimulated IL-6 production in Müller cells at a concentration as low as 10-12 M, which was not sufficient to induce cell proliferation. This elevation of IL-6 production was significantly inhibited by PACAP6-38. These data suggest that Müller cells are one of the target cells for PACAP, stimulating the release of IL-6, and providing a mechanism whereby PACAP exerts a significant neuroprotective effect in the retina.
AB - We have investigated the in vivo effect of PACAP on rat Müller cells that are the predominant glial element in the retina. Müller cells were treated with PACAP38, either alone or in the presence of the PACAP-selective antagonist, PACAP6-38. Cellular proliferation was determined by measuring the incorporation of bromodeoxyuridine, while interleukin-6 (IL-6) levels in the culture medium were examined using a B9 cell bioassay. In cultured rat Müller cells, the expression of PACAP receptor (PAC1-R) was assessed with immunohistochemistry using a PAC1-R-specific antiserum. PACAP stimulated IL-6 production in Müller cells at a concentration as low as 10-12 M, which was not sufficient to induce cell proliferation. This elevation of IL-6 production was significantly inhibited by PACAP6-38. These data suggest that Müller cells are one of the target cells for PACAP, stimulating the release of IL-6, and providing a mechanism whereby PACAP exerts a significant neuroprotective effect in the retina.
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U2 - 10.1196/annals.1317.043
DO - 10.1196/annals.1317.043
M3 - Article
C2 - 16888221
AN - SCOPUS:33746393666
SN - 0077-8923
VL - 1070
SP - 535
EP - 539
JO - Annals of the New York Academy of Sciences
JF - Annals of the New York Academy of Sciences
ER -