Padina ditristromatica sp. nov. and padina pavonicoides sp. nov. (Dictyotales, Phaeophyceae), two new species from the mediterranean sea based on morphological and molecular markers

Ni Ni Win, Takeaki Hanyuda, Stefano G.A. Draisma, Giovanni Furnari, Alexandre Meinesz, Hiroshi Kawai

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

A study of the genus Padina in the Mediterranean Sea, based on detailed morphological and molecular analyses using chloroplast rbcL and mitochondrial cox3 gene sequences, as well as RuBisCO spacer and partial rbcS sequences, revealed the presence of two new species, P. ditristromatica Ni-Ni-Win & H. Kawai and P. pavonicoides Ni-Ni-Win & H. Kawai. They are two to three-layered and three-layered species, respectively. Padina ditristromatica is characterized by a thallus composed of two cell layers from the marginal portion to the inrolled margin and a mixture of two and three layers in the other portions; heavy calcification on both surfaces of the thallus except for the hair lines; alternating hair lines that are spaced at unequal distances between the upper and lower surfaces; and broad indusiate oogonial and tetrasporangial sori forming broken lines or patches arranged in a concentric row, which are distally close to the hair lines and half immersed in the epidermis layer only on the lower surface. Padina pavonicoides is characterized by a thallus composed of three cell layers from the base to the marginal portion and two layers at the inrolled margin; alternating hair lines that are spaced at equal distances between the upper and lower surfaces; and indusiate oogonial and tetrasporangial sori forming patches located distal to the hair lines only on the lower surface. All molecular phylogenetic analyses indicated that the new species are closely related to P. pavonica, a common species in the Mediterranean Sea. However, the cox3 region could not be amplified for P. ditristromatica. Therefore, the RuBisCO spacer and partial rbcS were analysed for the Mediterranean specimens in order to confirm their identity as well as their closest relationships. The combined rbcL, RuBisCO spacer and partial rbcS data also support their genetic separation and show that P. pavonica is more closely related to P. pavonicoides than P. ditristromatica, as in other molecular analyses.

Original languageEnglish
Pages (from-to)327-341
Number of pages15
JournalEuropean Journal of Phycology
Volume46
Issue number4
DOIs
Publication statusPublished - Nov 1 2011

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Dictyotales
Phaeophyceae
hair
Mediterranean Sea
hairs
new species
genetic markers
thallus
calcification
epidermis (animal)
chloroplast
chloroplasts
Padina
marker
cells
phylogenetics
nucleotide sequences
gene
phylogeny

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Plant Science

Cite this

Padina ditristromatica sp. nov. and padina pavonicoides sp. nov. (Dictyotales, Phaeophyceae), two new species from the mediterranean sea based on morphological and molecular markers. / Win, Ni Ni; Hanyuda, Takeaki; Draisma, Stefano G.A.; Furnari, Giovanni; Meinesz, Alexandre; Kawai, Hiroshi.

In: European Journal of Phycology, Vol. 46, No. 4, 01.11.2011, p. 327-341.

Research output: Contribution to journalArticle

Win, Ni Ni ; Hanyuda, Takeaki ; Draisma, Stefano G.A. ; Furnari, Giovanni ; Meinesz, Alexandre ; Kawai, Hiroshi. / Padina ditristromatica sp. nov. and padina pavonicoides sp. nov. (Dictyotales, Phaeophyceae), two new species from the mediterranean sea based on morphological and molecular markers. In: European Journal of Phycology. 2011 ; Vol. 46, No. 4. pp. 327-341.
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