Paired box 7 inhibits differentiation in 3T3-L1 preadipocytes

Wakana Izumi, Yuko Takuma, Ryo Ebihara, Wataru Mizunoya, Ryuichi Tatsumi, Mako Nakamura

Research output: Contribution to journalArticle

1 Citation (Scopus)

Abstract

Myogenesis is precisely proceeded by myogenic regulatory factors. Myogenic stem cells are activated, proliferated and fused into a multinuclear myofiber. Pax7, paired box 7, one of the earliest markers during myogenesis. It has been reported that Pax7 regulates the muscle marker genes, Myf5 and MyoD toward differentiation. The possible roles of Pax7 in myogenic cells have been well researched. However, it has not yet been clarified if Pax7 itself is able to induce myogenic fate in nonmyogenic lineage cells. In this study, we performed experiments using stably expressed Pax7 in 3T3-L1 preadipocytes to elucidate if Pax7 inhibits adipogenesis. We found that Pax7 represses adipogenic markers and prevents differentiation. These cells showed decreased expression of PDGFRα, PPARγ and Fabp4 and inhibited forming lipid droplets.

Original languageEnglish
Pages (from-to)1214-1219
Number of pages6
JournalAnimal Science Journal
Volume89
Issue number8
DOIs
Publication statusPublished - Aug 2018

All Science Journal Classification (ASJC) codes

  • Agricultural and Biological Sciences(all)

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