Palladium bionanoparticles production from acidic Pd(II) solutions and spent catalyst leachate using acidophilic Fe(III)-reducing bacteria

Naoko Okibe, Daisuke Nakayama, Takahiro Matsumoto

Research output: Contribution to journalArticle

6 Citations (Scopus)

Abstract

The acidophilic, Fe(III)-reducing heterotrophic bacteria Acidocella aromatica PFBCT and Acidiphilium cryptum SJH were utilized to produce palladium (Pd) bionanoparticles via a simple 1-step microbiological reaction. Monosaccharide (or intracellular NADH)-dependent reactions lead to visualization of intra/extra-cellular enzymatic Pd(0) nucleation. Formic acid-dependent reactions proceeded via the first slow Pd(0) nucleation phase and the following autocatalytic Pd(II) reduction phase regardless of the presence or viability of the cells. However, use of active cells (with full enzymatic and membrane protein activities) at low formic acid concentration (5 mM) was critical to allow sufficient time for Pd(II) biosorption and the following enzymatic Pd(0) nucleation, which consequently enabled production of fine, dense and well-dispersed Pd(0) bionanoparticles. Differences of the resultant Pd(0) nanoparticles in size, density and localization between the two bacteria under each condition tested suggested different activity and location of enzymes and membrane “Pd(II) trafficking” proteins responsible for Pd(0) nucleation. Despite the inhibitory effect of leaching lixiviant and dissolved metal ions, Pd(0) bionanoparticles were effectively formed by active Ac. aromatica cells from both acidic synthetic Pd(II) solutions and from the actual spent catalyst leachates at equivalent 18–19 nm median size with comparable catalytic activity.

Original languageEnglish
Pages (from-to)1091-1100
Number of pages10
JournalExtremophiles
Volume21
Issue number6
DOIs
Publication statusPublished - Nov 1 2017

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Palladium
Bacteria
formic acid
Acidiphilium
Monosaccharides
Protein Transport
NAD
Nanoparticles
Cell Survival
Membrane Proteins
Metals
Ions

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Molecular Medicine

Cite this

Palladium bionanoparticles production from acidic Pd(II) solutions and spent catalyst leachate using acidophilic Fe(III)-reducing bacteria. / Okibe, Naoko; Nakayama, Daisuke; Matsumoto, Takahiro.

In: Extremophiles, Vol. 21, No. 6, 01.11.2017, p. 1091-1100.

Research output: Contribution to journalArticle

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