Palytoxin is a non-12-o-tetradecanoylphorbol-13-acetate type tumor promoter in two-stage mouse skin carcinogenesis

Hirota Fujiki, Masami Suganuma, Michie Nakayasu, Hiromi Hakii, Takahiko Horiuchi, Shozo Takayama, Takashi Sugimura

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Abstract

Palytoxin, which is a toxin with a molecular weight of 2681 daltons isolated from a marine coelenterate, is a potent skin irritant. However, it did not induce ornithine decarboxylase in mouse skin, or adhesion of human promyelocytic leukemia cells (HL-60). Moreover, it did not inhibit the specific binding of (3H)12-O-tetradecanoylphorbol-13-acetate (TPA) to a mouse skin particulate fraction or activate protein kinase C isolated from mouse brain in vitro. Since palytoxin showed strong irritation on mouse ear in one short-term screening test for a promoter, it was examined in a two-stage carcinogenesis experiment. The incidence of tumors in a group of mice treated with 7, 12-dimethylbenz(a)anthracene plus palytoxin was 62.5% in week 25. These tumors were identified histologically as seven papillonmas and one carcinoma. This paper reports the potent tumor-promoting activity of palytoxin, which is classified as a non-TPA-type tumor promoter.

Original languageEnglish
Pages (from-to)707-710
Number of pages4
JournalCarcinogenesis
Volume7
Issue number5
DOIs
Publication statusPublished - May 1 1986

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Tetradecanoylphorbol Acetate
Carcinogens
Carcinogenesis
Skin
Neoplasms
Ornithine Decarboxylase
Irritants
HL-60 Cells
Protein Kinase C
Ear
Leukemia
Molecular Weight
palytoxin
Carcinoma
Incidence
Brain

All Science Journal Classification (ASJC) codes

  • Cancer Research

Cite this

Palytoxin is a non-12-o-tetradecanoylphorbol-13-acetate type tumor promoter in two-stage mouse skin carcinogenesis. / Fujiki, Hirota; Suganuma, Masami; Nakayasu, Michie; Hakii, Hiromi; Horiuchi, Takahiko; Takayama, Shozo; Sugimura, Takashi.

In: Carcinogenesis, Vol. 7, No. 5, 01.05.1986, p. 707-710.

Research output: Contribution to journalArticle

Fujiki, Hirota ; Suganuma, Masami ; Nakayasu, Michie ; Hakii, Hiromi ; Horiuchi, Takahiko ; Takayama, Shozo ; Sugimura, Takashi. / Palytoxin is a non-12-o-tetradecanoylphorbol-13-acetate type tumor promoter in two-stage mouse skin carcinogenesis. In: Carcinogenesis. 1986 ; Vol. 7, No. 5. pp. 707-710.
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