Pancreastatin molecular forms in normal human plasma

Kitayama Noboru Kitayama, Tateishi Kayoko Tateishi, Funakoshi Akihiro Funakoshi, Miyasak Kyoko Miyasak, Shimazoe Takao Shimazoe, Kono Akira Kono, Iwamoto Naoko Iwamoto, Matsuoka Yuji Matsuoka

Research output: Contribution to journalArticle

19 Citations (Scopus)

Abstract

Circulating molecular forms with pancreastatin (PST)-like immunoreactivity in plasma from normal subjects were examined. An immunoreactive form corresponding to a human PST-like sequence [human chromogranin-A-(250-301)] (hPST-52) and a larger form (mol wt 15-21 kDa) were detected by gel filtration of plasma from normal subjects. On high performance liquid chromatography, predominant immunoreactive forms coeluted with the three larger forms which were purified from the xenograft of human pancreatic islet cell carcinoma cell line QGP-1N cells and with synthetic hPST-52. The fraction containing larger forms purified from xenograft of QGP-1N cells had biological activity equivalent to that of hPST-52 on the inhibition of pancreatic exocrine secretion. These results suggest that the larger molecular forms as well as hPST-52 may be physiologically important circulating forms of PST in human.

Original languageEnglish
Pages (from-to)1571-1578
Number of pages8
JournalLife Sciences
Volume54
Issue number21
DOIs
Publication statusPublished - 1994
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biochemistry, Genetics and Molecular Biology(all)
  • Pharmacology, Toxicology and Pharmaceutics(all)

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  • Cite this

    Noboru Kitayama, K., Kayoko Tateishi, T., Akihiro Funakoshi, F., Kyoko Miyasak, M., Takao Shimazoe, S., Akira Kono, K., Naoko Iwamoto, I., & Yuji Matsuoka, M. (1994). Pancreastatin molecular forms in normal human plasma. Life Sciences, 54(21), 1571-1578. https://doi.org/10.1016/0024-3205(94)90028-0