Parallel early development of zebrafish interrenal glands and pronephros: Differential control by wt1 and ff1b

Hwei Jan Hsu, Guang Lin, Bon Chu Chung

Research output: Contribution to journalReview article

75 Citations (Scopus)

Abstract

Steroids are synthesized mainly from the adrenal cortex. Adrenal deficiencies are often associated with problems related to its development, which is not fully understood. To better understand adrenocortical development, we studied zebrafish because of the ease of embryo manipulation. The adrenocortical equivalent in zebrafish is called the interrenal, because it is embedded in the kidney. We find that interrenal development parallels that of the embryonic kidney (pronephros). Primordial interrenal cells first appear as bilateral intermediate mesoderm expressing ff1b in a region ventral to the third somite. These cells then migrate toward the axial midline and fuse together. The pronephric primordia are wt1-expressing cells located next to the interrenal. They also migrate to the axial midline and fuse to become glomeruli at later developmental stages. Our gene knockdown experiments indicate that wt1 is required for its initial restricted expression in pronephric primordia, pronephric cell migration and fusion. wt1 also appears to be involved in interrenal development and ff1b expression. Similarly, ff1b is required for interrenal differentiation and activation of the differentiated gene, cyp11a1. Our results show that the zebrafish interrenal and pronephros are situated close together and go through parallel developmental processes but are governed by different signaling events.

Original languageEnglish
Pages (from-to)2107-2116
Number of pages10
JournalDevelopment
Volume130
Issue number10
DOIs
Publication statusPublished - May 1 2003

Fingerprint

Interrenal Gland
Pronephros
Zebrafish
Gene Knockdown Techniques
Kidney
Somites
Cell Fusion
Adrenal Cortex
Mesoderm
Transcriptional Activation
Embryonic Development
Cell Movement
Embryonic Structures
Steroids

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Cite this

Parallel early development of zebrafish interrenal glands and pronephros : Differential control by wt1 and ff1b. / Hsu, Hwei Jan; Lin, Guang; Chung, Bon Chu.

In: Development, Vol. 130, No. 10, 01.05.2003, p. 2107-2116.

Research output: Contribution to journalReview article

Hsu, Hwei Jan ; Lin, Guang ; Chung, Bon Chu. / Parallel early development of zebrafish interrenal glands and pronephros : Differential control by wt1 and ff1b. In: Development. 2003 ; Vol. 130, No. 10. pp. 2107-2116.
@article{96079754b97f4e48a29df3d40dcfc136,
title = "Parallel early development of zebrafish interrenal glands and pronephros: Differential control by wt1 and ff1b",
abstract = "Steroids are synthesized mainly from the adrenal cortex. Adrenal deficiencies are often associated with problems related to its development, which is not fully understood. To better understand adrenocortical development, we studied zebrafish because of the ease of embryo manipulation. The adrenocortical equivalent in zebrafish is called the interrenal, because it is embedded in the kidney. We find that interrenal development parallels that of the embryonic kidney (pronephros). Primordial interrenal cells first appear as bilateral intermediate mesoderm expressing ff1b in a region ventral to the third somite. These cells then migrate toward the axial midline and fuse together. The pronephric primordia are wt1-expressing cells located next to the interrenal. They also migrate to the axial midline and fuse to become glomeruli at later developmental stages. Our gene knockdown experiments indicate that wt1 is required for its initial restricted expression in pronephric primordia, pronephric cell migration and fusion. wt1 also appears to be involved in interrenal development and ff1b expression. Similarly, ff1b is required for interrenal differentiation and activation of the differentiated gene, cyp11a1. Our results show that the zebrafish interrenal and pronephros are situated close together and go through parallel developmental processes but are governed by different signaling events.",
author = "Hsu, {Hwei Jan} and Guang Lin and Chung, {Bon Chu}",
year = "2003",
month = "5",
day = "1",
doi = "10.1242/dev.00427",
language = "English",
volume = "130",
pages = "2107--2116",
journal = "Development (Cambridge)",
issn = "0950-1991",
publisher = "Company of Biologists Ltd",
number = "10",

}

TY - JOUR

T1 - Parallel early development of zebrafish interrenal glands and pronephros

T2 - Differential control by wt1 and ff1b

AU - Hsu, Hwei Jan

AU - Lin, Guang

AU - Chung, Bon Chu

PY - 2003/5/1

Y1 - 2003/5/1

N2 - Steroids are synthesized mainly from the adrenal cortex. Adrenal deficiencies are often associated with problems related to its development, which is not fully understood. To better understand adrenocortical development, we studied zebrafish because of the ease of embryo manipulation. The adrenocortical equivalent in zebrafish is called the interrenal, because it is embedded in the kidney. We find that interrenal development parallels that of the embryonic kidney (pronephros). Primordial interrenal cells first appear as bilateral intermediate mesoderm expressing ff1b in a region ventral to the third somite. These cells then migrate toward the axial midline and fuse together. The pronephric primordia are wt1-expressing cells located next to the interrenal. They also migrate to the axial midline and fuse to become glomeruli at later developmental stages. Our gene knockdown experiments indicate that wt1 is required for its initial restricted expression in pronephric primordia, pronephric cell migration and fusion. wt1 also appears to be involved in interrenal development and ff1b expression. Similarly, ff1b is required for interrenal differentiation and activation of the differentiated gene, cyp11a1. Our results show that the zebrafish interrenal and pronephros are situated close together and go through parallel developmental processes but are governed by different signaling events.

AB - Steroids are synthesized mainly from the adrenal cortex. Adrenal deficiencies are often associated with problems related to its development, which is not fully understood. To better understand adrenocortical development, we studied zebrafish because of the ease of embryo manipulation. The adrenocortical equivalent in zebrafish is called the interrenal, because it is embedded in the kidney. We find that interrenal development parallels that of the embryonic kidney (pronephros). Primordial interrenal cells first appear as bilateral intermediate mesoderm expressing ff1b in a region ventral to the third somite. These cells then migrate toward the axial midline and fuse together. The pronephric primordia are wt1-expressing cells located next to the interrenal. They also migrate to the axial midline and fuse to become glomeruli at later developmental stages. Our gene knockdown experiments indicate that wt1 is required for its initial restricted expression in pronephric primordia, pronephric cell migration and fusion. wt1 also appears to be involved in interrenal development and ff1b expression. Similarly, ff1b is required for interrenal differentiation and activation of the differentiated gene, cyp11a1. Our results show that the zebrafish interrenal and pronephros are situated close together and go through parallel developmental processes but are governed by different signaling events.

UR - http://www.scopus.com/inward/record.url?scp=0037736570&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0037736570&partnerID=8YFLogxK

U2 - 10.1242/dev.00427

DO - 10.1242/dev.00427

M3 - Review article

C2 - 12668625

AN - SCOPUS:0037736570

VL - 130

SP - 2107

EP - 2116

JO - Development (Cambridge)

JF - Development (Cambridge)

SN - 0950-1991

IS - 10

ER -