TY - JOUR
T1 - Parathyroid hormone 1 (1-34) acts on the scales and involves calcium metabolism in goldfish
AU - Suzuki, Nobuo
AU - Danks, Janine A.
AU - Maruyama, Yusuke
AU - Ikegame, Mika
AU - Sasayama, Yuichi
AU - Hattori, Atsuhiko
AU - Nakamura, Masahisa
AU - Tabata, Makoto J.
AU - Yamamoto, Toshio
AU - Furuya, Ryo
AU - Saijoh, Kiyofumi
AU - Mishima, Hiroyuki
AU - Srivastav, Ajai K.
AU - Furusawa, Yukihiro
AU - Kondo, Takashi
AU - Tabuchi, Yoshiaki
AU - Takasaki, Ichiro
AU - Chowdhury, Vishwajit S.
AU - Hayakawa, Kazuichi
AU - Martin, T. John
N1 - Funding Information:
This study was supported in part by grants to N.S. [Kurita Water and Environment Foundation; Grant-in-Aid for Scientific Research (C) No. 21500404 by JSPS ; Grant-in-Aid for Space Utilization by Japan Aerospace Exploration Agency ], to A.H. [Grant-in-Aid for Scientific Research (C) No. 21570062 by JSPS ], to H.M. [Grant-in-Aid for Scientific Research (C) No. 20592168 by JSPS ], and to K.H. [Grant-in-Aid for Scientific Research of ExTEND2005 and the Environment Research and Technology Development Fund (B-0905) by the Ministry of the Environment in Japan ; Health and Labour Sciences Research Grants of Ministry of Health, Labour and Welfare, Japan ; Grant-in-Aid for Scientific Research (B) No. 21390034 and for Exploratory Research No. 21651018 by JSPS ].
PY - 2011/5/1
Y1 - 2011/5/1
N2 - The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100. pg/ml-10. ng/ml) significantly increased ALP activity at 6. h of incubation. High-dose (10. ng/ml) fugu PTH1 significantly increased ALP activity even after 18. h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6. h of incubation, but fugu PTH1 (100. pg/ml-10. ng/ml) significantly increased TRAP activity at 18. h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-κB ligand in osteoblasts and the receptor activator NF-κB mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.
AB - The effect of fugu parathyroid hormone 1 (fugu PTH1) on osteoblasts and osteoclasts in teleosts was examined with an assay system using teleost scale and the following markers: alkaline phosphatase (ALP) for osteoblasts and tartrate-resistant acid phosphatase (TRAP) for osteoclasts. Synthetic fugu PTH1 (1-34) (100. pg/ml-10. ng/ml) significantly increased ALP activity at 6. h of incubation. High-dose (10. ng/ml) fugu PTH1 significantly increased ALP activity even after 18. h of incubation. In the case of TRAP activity, fugu PTH1 did not change at 6. h of incubation, but fugu PTH1 (100. pg/ml-10. ng/ml) significantly increased TRAP activity at 18. h. Similar results were obtained for human PTH (1-34), but there was an even greater response with fugu PTH1 than with human PTH. In vitro, we demonstrated that both the receptor activator of the NF-κB ligand in osteoblasts and the receptor activator NF-κB mRNA expression in osteoclasts increased significantly by fugu PTH1 treatment. In an in vivo experiment, fugu PTH1 induced hypercalcemia resulted from the increase of both osteoblastic and osteoclastic activities in the scale as well as the decrease of scale calcium contents after fugu PTH1 injection. In addition, an in vitro experiment with intramuscular autotransplanted scale indicated that the ratio of multinucleated osteoclasts/mononucleated osteoclasts in PTH-treated scales was significantly higher than that in the control scales. Thus, we concluded that PTH acts on osteoblasts and osteoclasts in the scales and regulates calcium metabolism in goldfish.
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U2 - 10.1016/j.bone.2011.02.004
DO - 10.1016/j.bone.2011.02.004
M3 - Article
C2 - 21334472
AN - SCOPUS:79954593765
SN - 8756-3282
VL - 48
SP - 1186
EP - 1193
JO - Bone
JF - Bone
IS - 5
ER -
