Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development

Ji Guang Liu; Makoto J. Tabata; Takafumi Fujii; Tomoharu Ohmori; Makoto Abe; Yasuyoshi Ohsaki; Joji Kato; Satoshi Wakisaka; Masahiro Iwamoto; Kojiro Kurisu

doi:10.1016/S0925-4773(00)00366-X

Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development

Ji Guang Liu, Makoto J. Tabata, Takafumi Fujii, Tomoharu Ohmori, Makoto Abe, Yasuyoshi Ohsaki, Joji Kato, Satoshi Wakisaka, Masahiro Iwamoto, Kojiro Kurisu

Oral Biological Sciences

Research output: Contribution to journal › Article › peer-review

25 Citations (Scopus)

Abstract

In order to elucidate the role of parathyroid hormone-related peptide (PTHrP) in tooth development, we treated tooth germ explants of mouse molars with antisense phosphorothioate-oligodeoxynucleotide (ODN) against PTHrP. Antisense ODN-treatment of the explants resulted in the invasion of the tooth germs by bone. The number of tartrate-resistant acid phosphatase (TRAP)-positive cells around the tooth germs in antisense ODN-treated explants was much lower than that of the control explants. Electron microscopic examination suggested that the antisense ODN-treatment inhibited differentiation of osteoclasts. Treatment of the explants with bisphosphonate or vitamin K2, inhibitors of the differentiation of osteoclasts, induced the invasion by bone into the tooth germs as observed in the antisense ODN-treated explants. The results obtained suggest that PTHrP is involved in the mechanism protecting tooth germs from bone invasion by promoting the differentiation of osteoclasts around them. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original language	English
Pages (from-to)	189-200
Number of pages	12
Journal	Mechanisms of Development
Volume	95
Issue number	1-2
DOIs	https://doi.org/10.1016/S0925-4773(00)00366-X
Publication status	Published - Jul 1 2000

All Science Journal Classification (ASJC) codes

Embryology
Developmental Biology

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Liu, J. G., Tabata, M. J., Fujii, T., Ohmori, T., Abe, M., Ohsaki, Y., Kato, J., Wakisaka, S., Iwamoto, M., & Kurisu, K. (2000). Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development. Mechanisms of Development, 95(1-2), 189-200. https://doi.org/10.1016/S0925-4773(00)00366-X

Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development. / Liu, Ji Guang; Tabata, Makoto J.; Fujii, Takafumi; Ohmori, Tomoharu; Abe, Makoto; Ohsaki, Yasuyoshi; Kato, Joji; Wakisaka, Satoshi; Iwamoto, Masahiro; Kurisu, Kojiro.

In: Mechanisms of Development, Vol. 95, No. 1-2, 01.07.2000, p. 189-200.

Research output: Contribution to journal › Article › peer-review

Liu, JG, Tabata, MJ, Fujii, T, Ohmori, T, Abe, M, Ohsaki, Y, Kato, J, Wakisaka, S, Iwamoto, M & Kurisu, K 2000, 'Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development', Mechanisms of Development, vol. 95, no. 1-2, pp. 189-200. https://doi.org/10.1016/S0925-4773(00)00366-X

Liu, Ji Guang ; Tabata, Makoto J. ; Fujii, Takafumi ; Ohmori, Tomoharu ; Abe, Makoto ; Ohsaki, Yasuyoshi ; Kato, Joji ; Wakisaka, Satoshi ; Iwamoto, Masahiro ; Kurisu, Kojiro. / Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development. In: Mechanisms of Development. 2000 ; Vol. 95, No. 1-2. pp. 189-200.

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title = "Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development",

abstract = "In order to elucidate the role of parathyroid hormone-related peptide (PTHrP) in tooth development, we treated tooth germ explants of mouse molars with antisense phosphorothioate-oligodeoxynucleotide (ODN) against PTHrP. Antisense ODN-treatment of the explants resulted in the invasion of the tooth germs by bone. The number of tartrate-resistant acid phosphatase (TRAP)-positive cells around the tooth germs in antisense ODN-treated explants was much lower than that of the control explants. Electron microscopic examination suggested that the antisense ODN-treatment inhibited differentiation of osteoclasts. Treatment of the explants with bisphosphonate or vitamin K2, inhibitors of the differentiation of osteoclasts, induced the invasion by bone into the tooth germs as observed in the antisense ODN-treated explants. The results obtained suggest that PTHrP is involved in the mechanism protecting tooth germs from bone invasion by promoting the differentiation of osteoclasts around them. Copyright (C) 2000 Elsevier Science Ireland Ltd.",

author = "Liu, {Ji Guang} and Tabata, {Makoto J.} and Takafumi Fujii and Tomoharu Ohmori and Makoto Abe and Yasuyoshi Ohsaki and Joji Kato and Satoshi Wakisaka and Masahiro Iwamoto and Kojiro Kurisu",

note = "Funding Information: We are indebted to Dr M. Kumegawa for his kind provision of antibody for cathepsin K. We are grateful to Messrs J. Imada and M. Hara of Osaka University Faculty of Dentistry for their helpful assistance. The authors are indebted to Novartis Farma AG, Basel, Swizterland, for providing of Aredia (bisphosphonate) and Eizai Pharmaceutical Co., Tokyo, Japan, for donating the vitamin K2. This work was supported by a Grant-in-Aid for Scientific Research from the Japanese Government (Project 08457479).",

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AU - Liu, Ji Guang

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AU - Ohmori, Tomoharu

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AU - Ohsaki, Yasuyoshi

AU - Kato, Joji

AU - Wakisaka, Satoshi

AU - Iwamoto, Masahiro

AU - Kurisu, Kojiro

N1 - Funding Information: We are indebted to Dr M. Kumegawa for his kind provision of antibody for cathepsin K. We are grateful to Messrs J. Imada and M. Hara of Osaka University Faculty of Dentistry for their helpful assistance. The authors are indebted to Novartis Farma AG, Basel, Swizterland, for providing of Aredia (bisphosphonate) and Eizai Pharmaceutical Co., Tokyo, Japan, for donating the vitamin K2. This work was supported by a Grant-in-Aid for Scientific Research from the Japanese Government (Project 08457479).

PY - 2000/7/1

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N2 - In order to elucidate the role of parathyroid hormone-related peptide (PTHrP) in tooth development, we treated tooth germ explants of mouse molars with antisense phosphorothioate-oligodeoxynucleotide (ODN) against PTHrP. Antisense ODN-treatment of the explants resulted in the invasion of the tooth germs by bone. The number of tartrate-resistant acid phosphatase (TRAP)-positive cells around the tooth germs in antisense ODN-treated explants was much lower than that of the control explants. Electron microscopic examination suggested that the antisense ODN-treatment inhibited differentiation of osteoclasts. Treatment of the explants with bisphosphonate or vitamin K2, inhibitors of the differentiation of osteoclasts, induced the invasion by bone into the tooth germs as observed in the antisense ODN-treated explants. The results obtained suggest that PTHrP is involved in the mechanism protecting tooth germs from bone invasion by promoting the differentiation of osteoclasts around them. Copyright (C) 2000 Elsevier Science Ireland Ltd.

AB - In order to elucidate the role of parathyroid hormone-related peptide (PTHrP) in tooth development, we treated tooth germ explants of mouse molars with antisense phosphorothioate-oligodeoxynucleotide (ODN) against PTHrP. Antisense ODN-treatment of the explants resulted in the invasion of the tooth germs by bone. The number of tartrate-resistant acid phosphatase (TRAP)-positive cells around the tooth germs in antisense ODN-treated explants was much lower than that of the control explants. Electron microscopic examination suggested that the antisense ODN-treatment inhibited differentiation of osteoclasts. Treatment of the explants with bisphosphonate or vitamin K2, inhibitors of the differentiation of osteoclasts, induced the invasion by bone into the tooth germs as observed in the antisense ODN-treated explants. The results obtained suggest that PTHrP is involved in the mechanism protecting tooth germs from bone invasion by promoting the differentiation of osteoclasts around them. Copyright (C) 2000 Elsevier Science Ireland Ltd.

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