Parathyroid hormone-related peptide is involved in protection against invasion of tooth germs by bone via promoting the differentiation of osteoclasts during tooth development

Ji Guang Liu, Makoto J. Tabata, Takafumi Fujii, Tomoharu Ohmori, Makoto Abe, Yasuyoshi Ohsaki, Joji Kato, Satoshi Wakisaka, Masahiro Iwamoto, Kojiro Kurisu

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In order to elucidate the role of parathyroid hormone-related peptide (PTHrP) in tooth development, we treated tooth germ explants of mouse molars with antisense phosphorothioate-oligodeoxynucleotide (ODN) against PTHrP. Antisense ODN-treatment of the explants resulted in the invasion of the tooth germs by bone. The number of tartrate-resistant acid phosphatase (TRAP)-positive cells around the tooth germs in antisense ODN-treated explants was much lower than that of the control explants. Electron microscopic examination suggested that the antisense ODN-treatment inhibited differentiation of osteoclasts. Treatment of the explants with bisphosphonate or vitamin K2, inhibitors of the differentiation of osteoclasts, induced the invasion by bone into the tooth germs as observed in the antisense ODN-treated explants. The results obtained suggest that PTHrP is involved in the mechanism protecting tooth germs from bone invasion by promoting the differentiation of osteoclasts around them. Copyright (C) 2000 Elsevier Science Ireland Ltd.

Original languageEnglish
Pages (from-to)189-200
Number of pages12
JournalMechanisms of Development
Issue number1-2
Publication statusPublished - Jul 1 2000


All Science Journal Classification (ASJC) codes

  • Embryology
  • Developmental Biology

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