Parental imprinting: Potentially active chromatin of the repressed maternal allele of the mouse insulin-like growth factor II (Igf2) gene

Hiroyuki Sasaki, Petei A. Jones, J. Richard Chaillet, Anne C. Ferguson-Smith, Sheila C. Barton, Wolf Reik, M. Azim Surani

Research output: Contribution to journalArticle

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Abstract

The mouse insulin-like growth factor II (Igf2) gene, which is located on distal chromosome 7 (Chr7), has been shown previously to undergo tissue-specific parental imprinting. This imprinting results in expression of Igf2 from the paternally inherited chromosome and repression of the maternally inherited allele in most tissues of the developing embryo. We are using embryos with the maternal duplication and paternal deficiency of distal Chr7 to characterize the mechanism that underlies repression of the maternal allele. We show that the chromatin of the 5′ region of the repressed Igf2 allele is potentially active for transcription rather than heterochromatic. In particular, a CpG island that comprises one of the two strong promoters is unmethylated at both parental alleles, and DNase I hypersensitive sites in and around the strong promoters are consistently present on both parental chromosomes. In agreement with the chromatin state, primary transcripts from the maternal Jgf2 allele have been detected at low but significant levels. These findings differ from observations in other instances of imprinting, namely, X-chromosome inactivation and transgene imprinting in mice. Although no parent-specific differences were detected in either DNA methylation or sensitivity to nucleases at these promoters, we have observed parental methylation differences in a region several kilobases upstream of the first exon. The differential activity of the parental Igf2 alleles could be achieved through epigenetic modifications situated outside the promoters or by subtle and yet unidentified modifications at the promoters.

Original languageEnglish
Pages (from-to)1843-1856
Number of pages14
JournalGenes and Development
Volume6
Issue number10
DOIs
Publication statusPublished - Jan 1 1992
Externally publishedYes

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Genomic Imprinting
Insulin-Like Growth Factor II
Chromatin
Alleles
Mothers
Genes
Chromosomes, Human, Pair 7
Embryonic Structures
Chromosomes
X Chromosome Inactivation
CpG Islands
Deoxyribonuclease I
DNA Methylation
Transgenes
Epigenomics
Methylation
Exons

All Science Journal Classification (ASJC) codes

  • Genetics
  • Developmental Biology

Cite this

Parental imprinting : Potentially active chromatin of the repressed maternal allele of the mouse insulin-like growth factor II (Igf2) gene. / Sasaki, Hiroyuki; Jones, Petei A.; Chaillet, J. Richard; Ferguson-Smith, Anne C.; Barton, Sheila C.; Reik, Wolf; Surani, M. Azim.

In: Genes and Development, Vol. 6, No. 10, 01.01.1992, p. 1843-1856.

Research output: Contribution to journalArticle

Sasaki, Hiroyuki ; Jones, Petei A. ; Chaillet, J. Richard ; Ferguson-Smith, Anne C. ; Barton, Sheila C. ; Reik, Wolf ; Surani, M. Azim. / Parental imprinting : Potentially active chromatin of the repressed maternal allele of the mouse insulin-like growth factor II (Igf2) gene. In: Genes and Development. 1992 ; Vol. 6, No. 10. pp. 1843-1856.
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