Parental methylation patterns of a transgenic locus in adult somatic tissues are imprinted during gametogenesis

T. Ueda, K. Yamazaki, R. Suzuki, H. Fujimoto, Hiroyuki Sasaki, Y. Sakaki, T. Higashinakagawa

Research output: Contribution to journalArticle

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Abstract

The methylation status fo a mouse metallothionein-I/human transthyretin fusion gene was studied during gametogenesis in transgenic mice. In the adult tissues of this mouse line, the promoter region of the transgene on chromosome 11 is methylated when it is maternally inherited and undermethylated when it is paternally inherited. Germ cells from various developmental stages of gametogenesis were isolated, and their DNAs were assayed using methylation-sensitive restricion endonucleases and the polymerase chain reaction. Only low to nonexistent levels of transgene methylation were detected in germ cells from 14.5-day-old male and female fetuses irrespective of the parental origin of the transgene. This undermethylated state persisted in oocytes from newborn females as well as in testicular spermatogenic cells and sperm. By contrast, the transgene promoter was completely methylated in fully grown oocytes arrested at the first meiotic prophase. The endogenous metallothionein-I gene promoter, located on a different chromosome, remained undermethylated at all stages examined, consistent with previous findings reported for a typical C(p)G island. Taken together, the results suggest that parental-specific adult patterns of transgene methylation are established during gametogenesis.

Original languageEnglish
Pages (from-to)831-839
Number of pages9
JournalDevelopment
Volume116
Issue number4
Publication statusPublished - Dec 1 1992

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Gametogenesis
Transgenes
Methylation
Metallothionein
Germ Cells
Oocytes
Prophase
Chromosomes, Human, Pair 11
Prealbumin
Endonucleases
Gene Fusion
Islands
Genetic Promoter Regions
Transgenic Mice
Spermatozoa
Fetus
Chromosomes
Polymerase Chain Reaction
DNA
Genes

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Developmental Biology

Cite this

Ueda, T., Yamazaki, K., Suzuki, R., Fujimoto, H., Sasaki, H., Sakaki, Y., & Higashinakagawa, T. (1992). Parental methylation patterns of a transgenic locus in adult somatic tissues are imprinted during gametogenesis. Development, 116(4), 831-839.

Parental methylation patterns of a transgenic locus in adult somatic tissues are imprinted during gametogenesis. / Ueda, T.; Yamazaki, K.; Suzuki, R.; Fujimoto, H.; Sasaki, Hiroyuki; Sakaki, Y.; Higashinakagawa, T.

In: Development, Vol. 116, No. 4, 01.12.1992, p. 831-839.

Research output: Contribution to journalArticle

Ueda, T, Yamazaki, K, Suzuki, R, Fujimoto, H, Sasaki, H, Sakaki, Y & Higashinakagawa, T 1992, 'Parental methylation patterns of a transgenic locus in adult somatic tissues are imprinted during gametogenesis', Development, vol. 116, no. 4, pp. 831-839.
Ueda T, Yamazaki K, Suzuki R, Fujimoto H, Sasaki H, Sakaki Y et al. Parental methylation patterns of a transgenic locus in adult somatic tissues are imprinted during gametogenesis. Development. 1992 Dec 1;116(4):831-839.
Ueda, T. ; Yamazaki, K. ; Suzuki, R. ; Fujimoto, H. ; Sasaki, Hiroyuki ; Sakaki, Y. ; Higashinakagawa, T. / Parental methylation patterns of a transgenic locus in adult somatic tissues are imprinted during gametogenesis. In: Development. 1992 ; Vol. 116, No. 4. pp. 831-839.
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