Partial amino acid sequences of two mitochondrial and two microsomal cytochrome p-450's from adrenal cortex

Tadashi Ogishima, Yoshiie Okada, Shiro Kominami, Shigeki Takemori, Tsuneo Omura

Research output: Contribution to journalArticle

18 Citations (Scopus)

Abstract

Partial amino acid sequences of two mitochondrial cytochrome P-450's, P-450 (SCC) and P-450 (11β), and two microsomal cytochrome P-450's, P-450 (C-21), and P-450 (17α, Lyase), from adrenal cortex were analyzed and compared. Mitochondrial P-450's and microsomal P-450's were different in the amino acid sequences at their NH2-terminals. The sequences of microsomal P-450's started from terminal meth-ionine and were highly hydrophobic, whereas those of mitochondrial P-450's lacked NH2-terminal methionine and were not hydrophobic. These findings strongly suggest that the NH2-terminal portions of newly synthesized P-450's determine their intracellular localization to different cell organelles.

Original languageEnglish
Pages (from-to)1711-1714
Number of pages4
JournalJournal of Biochemistry
Volume94
Issue number5
Publication statusPublished - Nov 1983

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Adrenal Cortex
Cytochromes
Cytochrome P-450 Enzyme System
Amino Acid Sequence
Amino Acids
Lyases
Methionine
Organelles

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

Cite this

Partial amino acid sequences of two mitochondrial and two microsomal cytochrome p-450's from adrenal cortex. / Ogishima, Tadashi; Okada, Yoshiie; Kominami, Shiro; Takemori, Shigeki; Omura, Tsuneo.

In: Journal of Biochemistry, Vol. 94, No. 5, 11.1983, p. 1711-1714.

Research output: Contribution to journalArticle

Ogishima, Tadashi ; Okada, Yoshiie ; Kominami, Shiro ; Takemori, Shigeki ; Omura, Tsuneo. / Partial amino acid sequences of two mitochondrial and two microsomal cytochrome p-450's from adrenal cortex. In: Journal of Biochemistry. 1983 ; Vol. 94, No. 5. pp. 1711-1714.
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