Partial amino acid sequences of two mitochondrial and two microsomal cytochrome p-450's from adrenal cortex

Tadashi Ogishima, Yoshiie Okada, Shiro Kominami, Shigeki Takemori, Tsuneo Omura

Research output: Contribution to journalArticlepeer-review

18 Citations (Scopus)

Abstract

Partial amino acid sequences of two mitochondrial cytochrome P-450's, P-450 (SCC) and P-450 (11β), and two microsomal cytochrome P-450's, P-450 (C-21), and P-450 (17α, Lyase), from adrenal cortex were analyzed and compared. Mitochondrial P-450's and microsomal P-450's were different in the amino acid sequences at their NH2-terminals. The sequences of microsomal P-450's started from terminal meth-ionine and were highly hydrophobic, whereas those of mitochondrial P-450's lacked NH2-terminal methionine and were not hydrophobic. These findings strongly suggest that the NH2-terminal portions of newly synthesized P-450's determine their intracellular localization to different cell organelles.

Original languageEnglish
Pages (from-to)1711-1714
Number of pages4
JournalJournal of Biochemistry
Volume94
Issue number5
Publication statusPublished - Nov 1983

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Molecular Biology

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