Partial purification and characterization of the fourth component (C4) of carp complement

Miki Nakao, Tomoki Yano, Hiroko Matsuyama

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

The fourth complement component (C4) of carp Cyprinus carpio was partially purified from serum by affinity chromatography on Blue-Cellulofine, ion-exchange chromatography on DEAE-Toyopearl 650M and gel filtration on Sepharose CL-6B using ammonia-inactivated carp serum as a reagent for detecting C4. About 1, 000-fold purification was achieved in a yield of 25% of the initial hemolytic activity. This partially purified C4 was free of C1 and C2, and it contained trace amounts of C3. The molecular weight of carp C4 was estimated to be 170, 000 by gel filtration on Sepharose CL-6B. The protein migrated to a slow β-globulin region on agarose gel electrophoresis. Carp C4 was stable on heating at 58°C for 60 min. Incubation with ammonia or hydrazine led to inactivation of the component, while carrageenan or zymosan had no effect. These characteristics of carp C4 were in fair agreement with those reported for mammalian C4. Carp C4 was, however, incompatible with C1 or C2 of the guinea pig complement.

Original languageEnglish
Pages (from-to)243-250
Number of pages8
JournalFish Pathology
Volume23
Issue number4
DOIs
Publication statusPublished - Jan 1 1988

Fingerprint

carp
purification
complement
gel
chromatography
serum
ammonia
agarose
carrageenan
pig
electrokinesis
ion exchange
incubation
gels
fold
hydrazine
heating
zymosan
protein
ion exchange chromatography

All Science Journal Classification (ASJC) codes

  • Aquatic Science
  • Animal Science and Zoology

Cite this

Partial purification and characterization of the fourth component (C4) of carp complement. / Nakao, Miki; Yano, Tomoki; Matsuyama, Hiroko.

In: Fish Pathology, Vol. 23, No. 4, 01.01.1988, p. 243-250.

Research output: Contribution to journalArticle

Nakao, Miki ; Yano, Tomoki ; Matsuyama, Hiroko. / Partial purification and characterization of the fourth component (C4) of carp complement. In: Fish Pathology. 1988 ; Vol. 23, No. 4. pp. 243-250.
@article{ed0d4c9356004dbca64da97553ef8550,
title = "Partial purification and characterization of the fourth component (C4) of carp complement",
abstract = "The fourth complement component (C4) of carp Cyprinus carpio was partially purified from serum by affinity chromatography on Blue-Cellulofine, ion-exchange chromatography on DEAE-Toyopearl 650M and gel filtration on Sepharose CL-6B using ammonia-inactivated carp serum as a reagent for detecting C4. About 1, 000-fold purification was achieved in a yield of 25{\%} of the initial hemolytic activity. This partially purified C4 was free of C1 and C2, and it contained trace amounts of C3. The molecular weight of carp C4 was estimated to be 170, 000 by gel filtration on Sepharose CL-6B. The protein migrated to a slow β-globulin region on agarose gel electrophoresis. Carp C4 was stable on heating at 58°C for 60 min. Incubation with ammonia or hydrazine led to inactivation of the component, while carrageenan or zymosan had no effect. These characteristics of carp C4 were in fair agreement with those reported for mammalian C4. Carp C4 was, however, incompatible with C1 or C2 of the guinea pig complement.",
author = "Miki Nakao and Tomoki Yano and Hiroko Matsuyama",
year = "1988",
month = "1",
day = "1",
doi = "10.3147/jsfp.23.243",
language = "English",
volume = "23",
pages = "243--250",
journal = "Fish Pathology",
issn = "0388-788X",
publisher = "Japanese Society of Fish Pathology",
number = "4",

}

TY - JOUR

T1 - Partial purification and characterization of the fourth component (C4) of carp complement

AU - Nakao, Miki

AU - Yano, Tomoki

AU - Matsuyama, Hiroko

PY - 1988/1/1

Y1 - 1988/1/1

N2 - The fourth complement component (C4) of carp Cyprinus carpio was partially purified from serum by affinity chromatography on Blue-Cellulofine, ion-exchange chromatography on DEAE-Toyopearl 650M and gel filtration on Sepharose CL-6B using ammonia-inactivated carp serum as a reagent for detecting C4. About 1, 000-fold purification was achieved in a yield of 25% of the initial hemolytic activity. This partially purified C4 was free of C1 and C2, and it contained trace amounts of C3. The molecular weight of carp C4 was estimated to be 170, 000 by gel filtration on Sepharose CL-6B. The protein migrated to a slow β-globulin region on agarose gel electrophoresis. Carp C4 was stable on heating at 58°C for 60 min. Incubation with ammonia or hydrazine led to inactivation of the component, while carrageenan or zymosan had no effect. These characteristics of carp C4 were in fair agreement with those reported for mammalian C4. Carp C4 was, however, incompatible with C1 or C2 of the guinea pig complement.

AB - The fourth complement component (C4) of carp Cyprinus carpio was partially purified from serum by affinity chromatography on Blue-Cellulofine, ion-exchange chromatography on DEAE-Toyopearl 650M and gel filtration on Sepharose CL-6B using ammonia-inactivated carp serum as a reagent for detecting C4. About 1, 000-fold purification was achieved in a yield of 25% of the initial hemolytic activity. This partially purified C4 was free of C1 and C2, and it contained trace amounts of C3. The molecular weight of carp C4 was estimated to be 170, 000 by gel filtration on Sepharose CL-6B. The protein migrated to a slow β-globulin region on agarose gel electrophoresis. Carp C4 was stable on heating at 58°C for 60 min. Incubation with ammonia or hydrazine led to inactivation of the component, while carrageenan or zymosan had no effect. These characteristics of carp C4 were in fair agreement with those reported for mammalian C4. Carp C4 was, however, incompatible with C1 or C2 of the guinea pig complement.

UR - http://www.scopus.com/inward/record.url?scp=0010382690&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0010382690&partnerID=8YFLogxK

U2 - 10.3147/jsfp.23.243

DO - 10.3147/jsfp.23.243

M3 - Article

AN - SCOPUS:0010382690

VL - 23

SP - 243

EP - 250

JO - Fish Pathology

JF - Fish Pathology

SN - 0388-788X

IS - 4

ER -