Partial purification of phosphoramidon-sensitive endothelin converting enzyme in porcine aortic endothelial cells: High affinity for Ricinus communis agglutinin

Keizo Ohnaka; Motoaki Nishikawa; Ryoichi Takayanagi; Masafumi Haji; Hajime Nawata

doi:10.1016/0006-291X(92)91668-G

Partial purification of phosphoramidon-sensitive endothelin converting enzyme in porcine aortic endothelial cells: High affinity for Ricinus communis agglutinin

Keizo Ohnaka, Motoaki Nishikawa, Ryoichi Takayanagi, Masafumi Haji, Hajime Nawata

Advanced Medical Intiatives

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Abstract

A membrane-bound endothelin converting enzyme (ECE) of porcine aortic endothelial cells (ECs) was solubilized with Lubrol PX with high efficiency and stability. The solubilized ECE was bound to Ricinus communis agglutinin (RCA) but not to peanut agglutinin (PNA) or wheat germ agglutinin (WGA), suggesting that the ECE has a galactosylated structure possessing a high affinity for RCA. The sequential chromatography on RCA-agarose, PNA-agarose and a TSKgel DEAE-5PW column attained 2,100-fold purification for the ECE over the membrane fractions. The purified ECE was sensitively inhibited by phosphoramidon but not by thiorphan. The present RCA and PNA affinity column procedures may be a powerful approach to isolation of ECE of EC origin.

Original language	English
Pages (from-to)	611-616
Number of pages	6
Journal	Biochemical and Biophysical Research Communications
Volume	185
Issue number	2
DOIs	https://doi.org/10.1016/0006-291X(92)91668-G
Publication status	Published - Jun 15 1992

All Science Journal Classification (ASJC) codes

Biophysics
Biochemistry
Molecular Biology
Cell Biology

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Partial purification of phosphoramidon-sensitive endothelin converting enzyme in porcine aortic endothelial cells : High affinity for Ricinus communis agglutinin. / Ohnaka, Keizo; Nishikawa, Motoaki; Takayanagi, Ryoichi et al.

In: Biochemical and Biophysical Research Communications, Vol. 185, No. 2, 15.06.1992, p. 611-616.

Research output: Contribution to journal › Article › peer-review

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AU - Nawata, Hajime

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Partial purification of phosphoramidon-sensitive endothelin converting enzyme in porcine aortic endothelial cells: High affinity for Ricinus communis agglutinin

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