Participation of cyclooxygenases in cutaneous thermal nociception under non-inflamed and inflamed conditions

M. Suzuki, I. Hayashi, Y. Nara, Y. Kumagai, H. Okamoto, Sumio Hoka, M. Majima

Research output: Contribution to journalArticle

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Abstract

Objective and Design: Effects of cyclooxygenase inhibitors on noxious thermal stimuli were investigated in non-inflamed and inflamed rats. Materials: Male Sprague-Dawley rats were used in this study. Treatment: Cyclooxygenase inhibitors, indomethacin, mofezolac, NS-398, and JTE-522 were administered orally at a dose of 10 mg/kg 1 h prior to and 4 h after the intravenous injection of lipopolysaccharide (1 mg/kg). Methods: The nociceptive response was evaluated from the escape latency of foot withdrawal to the thermal stimuli with a beam of light. Expression of cyclooxygenase was examined by reverse transcription-polymerase chain reaction. Results: In normal rat, administration of indomethacin, relatively cyclooxygenase-1-selective inhibitor, mofezolac, or cyclooxygenase-2-selective inhibitors, NS-398 and JTE-522 had no effects on the escape latency against thermal stimuli. Injection of lipopolysaccharide into rat induced the expression of mRNA for cyclooxygenase-2 in the subcutaneous tissue of foot pad. The escape latency at 8 h was significantly shortened by the injection. This hyperalgesia could be reversed by pretreatment of rat with NS-398 or JTE-522, but not with mofezolac. Conclusions: Cyclooxygenases may have little participation in peripheral skin thermal nociception in non-inflamed condition, although cyclooxygenase-2 could be responsible for the hyperalgesia during inflammation induced by lipopolysaccharide.

Original languageEnglish
Pages (from-to)283-287
Number of pages5
JournalInflammation Research
Volume50
Issue number5
DOIs
Publication statusPublished - May 29 2001

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Nociception
Prostaglandin-Endoperoxide Synthases
Hot Temperature
Lipopolysaccharides
Skin
Cyclooxygenase Inhibitors
Hyperalgesia
Cyclooxygenase 2
Indomethacin
Foot
Cyclooxygenase 1
Injections
Cyclooxygenase 2 Inhibitors
Subcutaneous Tissue
Intravenous Injections
Reverse Transcription
Sprague Dawley Rats
Inflammation
Light
Polymerase Chain Reaction

All Science Journal Classification (ASJC) codes

  • Immunology
  • Pharmacology

Cite this

Suzuki, M., Hayashi, I., Nara, Y., Kumagai, Y., Okamoto, H., Hoka, S., & Majima, M. (2001). Participation of cyclooxygenases in cutaneous thermal nociception under non-inflamed and inflamed conditions. Inflammation Research, 50(5), 283-287. https://doi.org/10.1007/s000110050755

Participation of cyclooxygenases in cutaneous thermal nociception under non-inflamed and inflamed conditions. / Suzuki, M.; Hayashi, I.; Nara, Y.; Kumagai, Y.; Okamoto, H.; Hoka, Sumio; Majima, M.

In: Inflammation Research, Vol. 50, No. 5, 29.05.2001, p. 283-287.

Research output: Contribution to journalArticle

Suzuki, M, Hayashi, I, Nara, Y, Kumagai, Y, Okamoto, H, Hoka, S & Majima, M 2001, 'Participation of cyclooxygenases in cutaneous thermal nociception under non-inflamed and inflamed conditions', Inflammation Research, vol. 50, no. 5, pp. 283-287. https://doi.org/10.1007/s000110050755
Suzuki, M. ; Hayashi, I. ; Nara, Y. ; Kumagai, Y. ; Okamoto, H. ; Hoka, Sumio ; Majima, M. / Participation of cyclooxygenases in cutaneous thermal nociception under non-inflamed and inflamed conditions. In: Inflammation Research. 2001 ; Vol. 50, No. 5. pp. 283-287.
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