Partition Coefficients of α-Amylase in the Aqueous Two-Phase System Containing Dextran and Polyethylene Glycol

Takeshi Furuya, Yoshio Iwai, Takashi Shimizu, Yasuhiko Arai

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7 Citations (Scopus)

Abstract

The liquid-liquid equilibria of the aqueous two-phase system containing dextran (Mn= 24, 200) and polyethylene glycol (Mn=3000) were measured at 20°C. The partition coefficients of α-amylase (molecular weight= 50,000) in this aqueous two-phase system were also determined at 20°C. Further, the liquid-liquid equilibria and the partition coefficients were correlated using the Flory-Huggins equation and the osmotic virial equation. It was found that the osmotic virial equation could give a better correlation.

Original languageEnglish
Pages (from-to)830-834
Number of pages5
Journalkagaku kogaku ronbunshu
Volume16
Issue number4
DOIs
Publication statusPublished - 1990

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

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