Partition coefficients of amino acids, peptides, and enzymes in dextran + poly(ethylene glycol) + water aqueous two-phase systems

Keijiro Kakisaka, Takashi Shindou, Toru Ishidao, Yoshio Iwai, Yasuhiko Arai

Research output: Contribution to journalArticle

4 Citations (Scopus)

Abstract

Partition coefficients are measured for five amino acids (aspartic acid, asparagine, methionine, cysteine and histidine) and two peptides (glycyl-glycine and hexa-glycine) in dextran + poly(ethylene glycol) + water aqueous two-phase system. The partition coefficients of the amino acids and peptides are correlated using the osmotic virial equation. The interaction coefficients contained in the equation can be calculated by hydrophilic group parameters. The partition coefficients of α-amylase calculated by the osmotic virial equation with the hydrophilic group parameters are in fairly good agreement with the experimental data, though a relatively large discrepancy is shown for β-amylase.

Original languageEnglish
Pages (from-to)991-995
Number of pages5
JournalJournal of Chemical Engineering of Japan
Volume31
Issue number6
DOIs
Publication statusPublished - Jan 1 1998

Fingerprint

Dextran
Amylases
Dextrans
Peptides
Polyethylene glycols
Amino acids
Enzymes
Glycylglycine
Amino Acids
Water
Asparagine
Histidine
Aspartic Acid
Methionine
Glycine
Cysteine
Acids

All Science Journal Classification (ASJC) codes

  • Chemistry(all)
  • Chemical Engineering(all)

Cite this

Partition coefficients of amino acids, peptides, and enzymes in dextran + poly(ethylene glycol) + water aqueous two-phase systems. / Kakisaka, Keijiro; Shindou, Takashi; Ishidao, Toru; Iwai, Yoshio; Arai, Yasuhiko.

In: Journal of Chemical Engineering of Japan, Vol. 31, No. 6, 01.01.1998, p. 991-995.

Research output: Contribution to journalArticle

@article{67b28257a00d4ca4bf17e611ee1dac96,
title = "Partition coefficients of amino acids, peptides, and enzymes in dextran + poly(ethylene glycol) + water aqueous two-phase systems",
abstract = "Partition coefficients are measured for five amino acids (aspartic acid, asparagine, methionine, cysteine and histidine) and two peptides (glycyl-glycine and hexa-glycine) in dextran + poly(ethylene glycol) + water aqueous two-phase system. The partition coefficients of the amino acids and peptides are correlated using the osmotic virial equation. The interaction coefficients contained in the equation can be calculated by hydrophilic group parameters. The partition coefficients of α-amylase calculated by the osmotic virial equation with the hydrophilic group parameters are in fairly good agreement with the experimental data, though a relatively large discrepancy is shown for β-amylase.",
author = "Keijiro Kakisaka and Takashi Shindou and Toru Ishidao and Yoshio Iwai and Yasuhiko Arai",
year = "1998",
month = "1",
day = "1",
doi = "10.1252/jcej.31.991",
language = "English",
volume = "31",
pages = "991--995",
journal = "Journal of Chemical Engineering of Japan",
issn = "0021-9592",
publisher = "Society of Chemical Engineers, Japan",
number = "6",

}

TY - JOUR

T1 - Partition coefficients of amino acids, peptides, and enzymes in dextran + poly(ethylene glycol) + water aqueous two-phase systems

AU - Kakisaka, Keijiro

AU - Shindou, Takashi

AU - Ishidao, Toru

AU - Iwai, Yoshio

AU - Arai, Yasuhiko

PY - 1998/1/1

Y1 - 1998/1/1

N2 - Partition coefficients are measured for five amino acids (aspartic acid, asparagine, methionine, cysteine and histidine) and two peptides (glycyl-glycine and hexa-glycine) in dextran + poly(ethylene glycol) + water aqueous two-phase system. The partition coefficients of the amino acids and peptides are correlated using the osmotic virial equation. The interaction coefficients contained in the equation can be calculated by hydrophilic group parameters. The partition coefficients of α-amylase calculated by the osmotic virial equation with the hydrophilic group parameters are in fairly good agreement with the experimental data, though a relatively large discrepancy is shown for β-amylase.

AB - Partition coefficients are measured for five amino acids (aspartic acid, asparagine, methionine, cysteine and histidine) and two peptides (glycyl-glycine and hexa-glycine) in dextran + poly(ethylene glycol) + water aqueous two-phase system. The partition coefficients of the amino acids and peptides are correlated using the osmotic virial equation. The interaction coefficients contained in the equation can be calculated by hydrophilic group parameters. The partition coefficients of α-amylase calculated by the osmotic virial equation with the hydrophilic group parameters are in fairly good agreement with the experimental data, though a relatively large discrepancy is shown for β-amylase.

UR - http://www.scopus.com/inward/record.url?scp=0032435164&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0032435164&partnerID=8YFLogxK

U2 - 10.1252/jcej.31.991

DO - 10.1252/jcej.31.991

M3 - Article

VL - 31

SP - 991

EP - 995

JO - Journal of Chemical Engineering of Japan

JF - Journal of Chemical Engineering of Japan

SN - 0021-9592

IS - 6

ER -