TY - JOUR
T1 - Pathogenesis of Vibrio parahaemolyticus
T2 - Intraperitoneal and Orogastric Challenge Experiments in Mice
AU - Hoashi, Kikuo
AU - Taniguchi, Hatsumi
AU - Yamashita, Hiroto
AU - Tsuji, Koichi
AU - Ogata, Kikuyo
AU - Mizuguchi, Yasuo
AU - Ohtomo, Nobuya
PY - 1990
Y1 - 1990
N2 - It has been noted that V. parahaemolyticus isolated from patients with food poisoning are almost always thermostable direct hemolysin (TDH)-positive, whereas, isolates from foods or environmental sources are usually TDH-negative. The virulence of V. parahaemolyticus in mice was examined by using intraperitoneal and orogastric challenge models, where the strains used were those isolated from patients and foods with food poisoning and included TDH-positive and -negative. The LD50 in mice was estimated to be approximately 107 in the former and 108 in the latter challenge model. In the mice killed by challenge, either intraperitoneal or orogastric, the following pathological changes were almost always observed: swelling, redness and fluid accumulation in the small intestine, particularly the upper part of it. Histologically, congestion, edema, and vacuolation were observed in mucosal and submucosal tissues; furthermore, ulceration at tips of some villi was noticed. The lethality and pathological finding in mice due to V. parahaemolyticus were demonstrated similarly in both challenge models and irrespective of the TDH phenotype of the strain used. It will be suggested that the findings in challenged mice may be attributed to enteropathogenic factor(s) other than TDH of V. parahaemolyticus.
AB - It has been noted that V. parahaemolyticus isolated from patients with food poisoning are almost always thermostable direct hemolysin (TDH)-positive, whereas, isolates from foods or environmental sources are usually TDH-negative. The virulence of V. parahaemolyticus in mice was examined by using intraperitoneal and orogastric challenge models, where the strains used were those isolated from patients and foods with food poisoning and included TDH-positive and -negative. The LD50 in mice was estimated to be approximately 107 in the former and 108 in the latter challenge model. In the mice killed by challenge, either intraperitoneal or orogastric, the following pathological changes were almost always observed: swelling, redness and fluid accumulation in the small intestine, particularly the upper part of it. Histologically, congestion, edema, and vacuolation were observed in mucosal and submucosal tissues; furthermore, ulceration at tips of some villi was noticed. The lethality and pathological finding in mice due to V. parahaemolyticus were demonstrated similarly in both challenge models and irrespective of the TDH phenotype of the strain used. It will be suggested that the findings in challenged mice may be attributed to enteropathogenic factor(s) other than TDH of V. parahaemolyticus.
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U2 - 10.1111/j.1348-0421.1990.tb01016.x
DO - 10.1111/j.1348-0421.1990.tb01016.x
M3 - Article
C2 - 2362562
AN - SCOPUS:0025279777
VL - 34
SP - 355
EP - 366
JO - Microbiology and Immunology
JF - Microbiology and Immunology
SN - 0385-5600
IS - 4
ER -