Pathogenesis of Vibrio parahaemolyticus: Intraperitoneal and Orogastric Challenge Experiments in Mice

Kikuo Hoashi, Hatsumi Taniguchi, Hiroto Yamashita, Koichi Tsuji, Kikuyo Ogata, Yasuo Mizuguchi, Nobuya Ohtomo

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20 Citations (Scopus)

Abstract

It has been noted that V. parahaemolyticus isolated from patients with food poisoning are almost always thermostable direct hemolysin (TDH)-positive, whereas, isolates from foods or environmental sources are usually TDH-negative. The virulence of V. parahaemolyticus in mice was examined by using intraperitoneal and orogastric challenge models, where the strains used were those isolated from patients and foods with food poisoning and included TDH-positive and -negative. The LD50 in mice was estimated to be approximately 107 in the former and 108 in the latter challenge model. In the mice killed by challenge, either intraperitoneal or orogastric, the following pathological changes were almost always observed: swelling, redness and fluid accumulation in the small intestine, particularly the upper part of it. Histologically, congestion, edema, and vacuolation were observed in mucosal and submucosal tissues; furthermore, ulceration at tips of some villi was noticed. The lethality and pathological finding in mice due to V. parahaemolyticus were demonstrated similarly in both challenge models and irrespective of the TDH phenotype of the strain used. It will be suggested that the findings in challenged mice may be attributed to enteropathogenic factor(s) other than TDH of V. parahaemolyticus.

Original languageEnglish
Pages (from-to)355-366
Number of pages12
JournalMICROBIOLOGY and IMMUNOLOGY
Volume34
Issue number4
DOIs
Publication statusPublished - 1990

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Immunology
  • Virology

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    Hoashi, K., Taniguchi, H., Yamashita, H., Tsuji, K., Ogata, K., Mizuguchi, Y., & Ohtomo, N. (1990). Pathogenesis of Vibrio parahaemolyticus: Intraperitoneal and Orogastric Challenge Experiments in Mice. MICROBIOLOGY and IMMUNOLOGY, 34(4), 355-366. https://doi.org/10.1111/j.1348-0421.1990.tb01016.x