Pax 2 expression in mesodermal segmentation and its relationship with EphA4 and Lunatic-fringe during chicken somitogenesis

Rinako Suetsugu, Yuki Sato, Yoshiko Takahashi

Research output: Contribution to journalArticle

2 Citations (Scopus)

Abstract

In the Pax gene family, which encodes DNA-binding proteins, Pax 2 has been known to play important roles in the formation of the midbrain/hindbrain boundary, eye, inner ear and kidney in vertebrates (Bioessays 19 (1997) 755). In this article, we report a segmentally regulated pattern of Pax 2 expression during chicken somitogenesis. Pax 2 mRNA is localized in the rostral end of the unsegmented presomitic mesoderm (PSM), abutting anteriorly on a prospective segmentation border. This pattern repeats every segmentation cycle (90 min) observed in ovo and also in the half embryo culture assay in which one half of PSM along the midline is fixed immediately while the other half is cultured for a given period. We also determined the sequence of changes in Pax 2 expression during a segmentation cycle by comparing the pattern of Pax 2 with that of Lunatic-fringe (L-fringe), known to cycle periodically in posterior PSM. A systematic comparison of the expression patterns between Pax 2, L-fringe and EphA4 further highlighted a close relationship between EphA4 and Pax 2 during a segmentation cycle. Lastly, Pax 2 is not segmentally expressed in mouse PSM, suggestive of species (avian)-specific mechanisms underlying somitic segmentation.

Original languageEnglish
Pages (from-to)157-161
Number of pages5
JournalGene Expression Patterns
Volume2
Issue number1-2
DOIs
Publication statusPublished - Nov 1 2002
Externally publishedYes

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Mesoderm
Chickens
Rhombencephalon
DNA-Binding Proteins
Inner Ear
Mesencephalon
Vertebrates
Embryonic Structures
Kidney
Messenger RNA
Genes

All Science Journal Classification (ASJC) codes

  • Genetics
  • Molecular Biology
  • Cellular and Molecular Neuroscience
  • Developmental Neuroscience

Cite this

Pax 2 expression in mesodermal segmentation and its relationship with EphA4 and Lunatic-fringe during chicken somitogenesis. / Suetsugu, Rinako; Sato, Yuki; Takahashi, Yoshiko.

In: Gene Expression Patterns, Vol. 2, No. 1-2, 01.11.2002, p. 157-161.

Research output: Contribution to journalArticle

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