@article{1c429380f03e4818977b5f40af20cd31,
title = "PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes",
abstract = "The ring finger protein PCGF6 (polycomb group ring finger 6) interacts with RING1A/B and E2F6 associated factors to form a non-canonical PRC1 (polycomb repressive complex 1) known as PCGF6-PRC1. Here, we demonstrate that PCGF6-PRC1 plays a role in repressing a subset of PRC1 target genes by recruiting RING1B and mediating downstream mono-ubiquitination of histone H2A. PCGF6-PRC1 bound loci are highly enriched for promoters of germ cell-related genes in mouse embryonic stem cells (ESCs). Conditional ablation of Pcgf6 in ESCs leads to robust derepression of such germ cell-related genes, in turn affecting cell growth and viability. We also find a role for PCGF6 in pre- and peri-implantation mouse embryonic development. We further show that a heterodimer of the transcription factors MAX and MGA recruits PCGF6 to target loci. PCGF6 thus links sequence specific target recognition by the MAX/MGA complex to PRC1-dependent transcriptional silencing of germ cell-specific genes in pluripotent stem cells.",
author = "Mitsuhiro Endoh and Endo, {Takaho A.} and Jun Shinga and Katsuhiko Hayashi and Anca Farcas and Ma, {Kit Wan} and Shinsuke Ito and Jafar Sharif and Tamie Endoh and Naoko Onaga and Manabu Nakayama and Tomoyuki Ishikura and Osamu Masui and Kessler, {Benedikt M.} and Toshio Suda and Osamu Ohara and Akihiko Okuda and Robert Klose and Haruhiko Koseki",
note = "Funding Information: We thank Dr. Natsumi Shimizu in RIKEN CDB for providing technical advice for mouse early embryos. This work was supported by Grant-in-Aid for Scientific Research on Innovative Areas (#26112516 to ME), Grant-in-Aid for Young Scientist (B) (# 25871129 to ME) and Grant-in-Aid for Scientific Research (C) (#16K07372 to ME) from Ministry of Education, Culture, Sports, Science and Technology (MEXT) of Japan. HK is a recipient of the Strategic Basic Research Programs, CREST grant. Ministry of Education, Culture, Sports, Science, and Technology Grant-in-Aid for Young Scientist (B) (#25871129) Mitsuhiro Endoh Ministry of Education, Culture, Sports, Science, and Technology Grant-in-Aid for Scientific Research (C) (#16K07372) Mitsuhiro Endoh Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Scientific Research on Innovative Areas (#26112516) Mitsuhiro Endoh Ministry of Education, Culture, Sports, Science and Technology Grant-in-Aid for Scientific Research on Innovative Areas (#24116711) Mitsuhiro Endoh RIKEN Haruhiko Koseki Ministry of Education, Culture, Sports, Science, and Technology Haruhiko Koseki Japan Science and Technology Agency Strategic Basic Research ProgramsHaruhiko Koseki The funders had no role in study design, data collection and interpretation, or the decision to submit the work for publication. Publisher Copyright: {\textcopyright} Endoh et al.",
year = "2017",
month = mar,
day = "17",
doi = "10.7554/eLife.21064",
language = "English",
volume = "6",
journal = "eLife",
issn = "2050-084X",
publisher = "eLife Sciences Publications",
}