Abstract
The ring finger protein PCGF6 (polycomb group ring finger 6) interacts with RING1A/B and E2F6 associated factors to form a non-canonical PRC1 (polycomb repressive complex 1) known as PCGF6-PRC1. Here, we demonstrate that PCGF6-PRC1 plays a role in repressing a subset of PRC1 target genes by recruiting RING1B and mediating downstream mono-ubiquitination of histone H2A. PCGF6-PRC1 bound loci are highly enriched for promoters of germ cell-related genes in mouse embryonic stem cells (ESCs). Conditional ablation of Pcgf6 in ESCs leads to robust derepression of such germ cell-related genes, in turn affecting cell growth and viability. We also find a role for PCGF6 in pre- and peri-implantation mouse embryonic development. We further show that a heterodimer of the transcription factors MAX and MGA recruits PCGF6 to target loci. PCGF6 thus links sequence specific target recognition by the MAX/MGA complex to PRC1-dependent transcriptional silencing of germ cell-specific genes in pluripotent stem cells.
| Original language | English |
|---|---|
| Article number | e21064 |
| Journal | eLife |
| Volume | 6 |
| DOIs | |
| Publication status | Published - Mar 17 2017 |
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All Science Journal Classification (ASJC) codes
- Neuroscience(all)
- Immunology and Microbiology(all)
- Biochemistry, Genetics and Molecular Biology(all)
Cite this
PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes. / Endoh, Mitsuhiro; Endo, Takaho A.; Shinga, Jun; Hayashi, Katsuhiko; Farcas, Anca; Ma, Kit Wan; Ito, Shinsuke; Sharif, Jafar; Endoh, Tamie; Onaga, Naoko; Nakayama, Manabu; Ishikura, Tomoyuki; Masui, Osamu; Kessler, Benedikt M.; Suda, Toshio; Ohara, Osamu; Okuda, Akihiko; Klose, Robert; Koseki, Haruhiko.
In: eLife, Vol. 6, e21064, 17.03.2017.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - PCGF6-PRC1 suppresses premature differentiation of mouse embryonic stem cells by regulating germ cell-related genes
AU - Endoh, Mitsuhiro
AU - Endo, Takaho A.
AU - Shinga, Jun
AU - Hayashi, Katsuhiko
AU - Farcas, Anca
AU - Ma, Kit Wan
AU - Ito, Shinsuke
AU - Sharif, Jafar
AU - Endoh, Tamie
AU - Onaga, Naoko
AU - Nakayama, Manabu
AU - Ishikura, Tomoyuki
AU - Masui, Osamu
AU - Kessler, Benedikt M.
AU - Suda, Toshio
AU - Ohara, Osamu
AU - Okuda, Akihiko
AU - Klose, Robert
AU - Koseki, Haruhiko
PY - 2017/3/17
Y1 - 2017/3/17
N2 - The ring finger protein PCGF6 (polycomb group ring finger 6) interacts with RING1A/B and E2F6 associated factors to form a non-canonical PRC1 (polycomb repressive complex 1) known as PCGF6-PRC1. Here, we demonstrate that PCGF6-PRC1 plays a role in repressing a subset of PRC1 target genes by recruiting RING1B and mediating downstream mono-ubiquitination of histone H2A. PCGF6-PRC1 bound loci are highly enriched for promoters of germ cell-related genes in mouse embryonic stem cells (ESCs). Conditional ablation of Pcgf6 in ESCs leads to robust derepression of such germ cell-related genes, in turn affecting cell growth and viability. We also find a role for PCGF6 in pre- and peri-implantation mouse embryonic development. We further show that a heterodimer of the transcription factors MAX and MGA recruits PCGF6 to target loci. PCGF6 thus links sequence specific target recognition by the MAX/MGA complex to PRC1-dependent transcriptional silencing of germ cell-specific genes in pluripotent stem cells.
AB - The ring finger protein PCGF6 (polycomb group ring finger 6) interacts with RING1A/B and E2F6 associated factors to form a non-canonical PRC1 (polycomb repressive complex 1) known as PCGF6-PRC1. Here, we demonstrate that PCGF6-PRC1 plays a role in repressing a subset of PRC1 target genes by recruiting RING1B and mediating downstream mono-ubiquitination of histone H2A. PCGF6-PRC1 bound loci are highly enriched for promoters of germ cell-related genes in mouse embryonic stem cells (ESCs). Conditional ablation of Pcgf6 in ESCs leads to robust derepression of such germ cell-related genes, in turn affecting cell growth and viability. We also find a role for PCGF6 in pre- and peri-implantation mouse embryonic development. We further show that a heterodimer of the transcription factors MAX and MGA recruits PCGF6 to target loci. PCGF6 thus links sequence specific target recognition by the MAX/MGA complex to PRC1-dependent transcriptional silencing of germ cell-specific genes in pluripotent stem cells.
UR - http://www.scopus.com/inward/record.url?scp=85017139635&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=85017139635&partnerID=8YFLogxK
U2 - 10.7554/eLife.21064
DO - 10.7554/eLife.21064
M3 - Article
C2 - 28304275
AN - SCOPUS:85017139635
VL - 6
JO - eLife
JF - eLife
SN - 2050-084X
M1 - e21064
ER -