PCR-RFLP Analysis of Amplified 28S Ribosomal DNA for Identification of Rhizoctonia spp., the Causal Agents of Sheath Diseases of Rice Plants

Masaru Matsumoto, Naruto Furuya, Nobuaki Matsuyama

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

Isolates of five Rhizoctonia spp., the causal agents of sheath disease of rice plants, were characterized by the restriction fragment length polymorphisms (RFLPs) analysis of a 28S ribosomal DNA (rDNA) gene amplified by the polymerase chain reaction (PCR). PCR-amplified products specific to AG 1-IA and AG 2-2 of R. solani, WAG-O of R. oryzae, AG-Ba of R. fumigata and AG-Bb of R. oryzae-sativae were selected and used for RFLP analysis. RFLP profiles obtained after digestion of 28S rDNA with the restriction enzymes were different depending on the four enzymes, HpaII, HhaI, Sau3AI and HaeIII, used. The RFLP profiles by the digestion with HpaII seemed to be useful for the benchmarks to discriminate Rhizoctonia spp.

Original languageEnglish
Pages (from-to)39-44
Number of pages6
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume41
Issue number1-2
Publication statusPublished - Dec 1 1996

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Rhizoctonia
Plant Diseases
Ribosomal DNA
Restriction Fragment Length Polymorphisms
ribosomal DNA
restriction fragment length polymorphism
polymerase chain reaction
rice
Polymerase Chain Reaction
Oryza
Digestion
digestion
Benchmarking
DNA Restriction Enzymes
enzymes
Enzymes
Genes
genes

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

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abstract = "Isolates of five Rhizoctonia spp., the causal agents of sheath disease of rice plants, were characterized by the restriction fragment length polymorphisms (RFLPs) analysis of a 28S ribosomal DNA (rDNA) gene amplified by the polymerase chain reaction (PCR). PCR-amplified products specific to AG 1-IA and AG 2-2 of R. solani, WAG-O of R. oryzae, AG-Ba of R. fumigata and AG-Bb of R. oryzae-sativae were selected and used for RFLP analysis. RFLP profiles obtained after digestion of 28S rDNA with the restriction enzymes were different depending on the four enzymes, HpaII, HhaI, Sau3AI and HaeIII, used. The RFLP profiles by the digestion with HpaII seemed to be useful for the benchmarks to discriminate Rhizoctonia spp.",
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