PCR-RFLP analysis of cpDNA in the genus Abies

L. Parducci, Alfred Szmidt

Research output: Contribution to journalArticle

67 Citations (Scopus)

Abstract

We used PCR-RFLP analysis of the chloroplast DNA of the genus Abies (family Pinaceae), to determine if the method could be employed to detect inter-specific variation in this genus and to study how the variation was distributed in different regions of the genome. Ten different chloroplast DNA regions, consisting of coding and non-coding DNA sequences, were amplified with specific primers in ten different Abies taxa. The amplification products were digested with several restriction enzymes. The results showed that the chloroplast genome is highly variable in most of the investigated taxa and contains multiple variable regions that appear to be distributed throughout the whole genome. Species-diagnostic markers were found for four of the ten investigated species. Unexpectedly, intra-specific variation was also detected in four species. It is likely that further studies, including larger sample sizes and/or more powerful methods for the detection of chloroplast DNA variation, will reveal additional variation for this genus.

Original languageEnglish
Pages (from-to)802-808
Number of pages7
JournalTheoretical and Applied Genetics
Volume98
Issue number5
DOIs
Publication statusPublished - Jan 1 1999

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Abies
Chloroplast DNA
chloroplast DNA
Restriction Fragment Length Polymorphisms
restriction fragment length polymorphism
Polymerase Chain Reaction
Pinaceae
Genome
Chloroplast Genome
genome
intergenic DNA
interspecific variation
Sample Size
nucleotide sequences
Enzymes
enzymes
methodology
sampling

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science
  • Genetics

Cite this

PCR-RFLP analysis of cpDNA in the genus Abies. / Parducci, L.; Szmidt, Alfred.

In: Theoretical and Applied Genetics, Vol. 98, No. 5, 01.01.1999, p. 802-808.

Research output: Contribution to journalArticle

Parducci, L. ; Szmidt, Alfred. / PCR-RFLP analysis of cpDNA in the genus Abies. In: Theoretical and Applied Genetics. 1999 ; Vol. 98, No. 5. pp. 802-808.
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