PDGF receptor β signaling in pericytes following ischemic brain injury

Koichi Arimura, Tetsuro Ago, Masahiro Kamouchi, Kuniyuki Nakamura, Koji Ishitsuka, Junya Kuroda, Hiroshi Sugimori, Hiroaki Ooboshi, Tomio Sasaki, Takanari Kitazono

Research output: Contribution to journalArticle

57 Citations (Scopus)

Abstract

Platelet derived growth factor (PDGF)-B plays a neuroprotective role in brain damages, including ischemic stroke. It has been suggested recently that PDGF receptor β (PDGFRβ) expressed in brain pericytes as well as in neurons and astrocytes may mediate the neuroprotective role of PDGF-B. The aims of this study were to elucidate the roles of PDGFRβ signaling in brain pericytes after ischemic stroke. In a rat middle cerebral artery occlusion (MCAO) model, PDGFRβ expression was induced specifically in the pericytes in peri-infarct areas and its level was gradually increased. PDGF-B induced marked phosphorylation of Akt in cultured brain pericytes. Consistently, PDGF-B was upregulated in endothelial cells in per-infarct areas and Akt was strongly phosphorylated in the PDGFRβ-expressing pericytes in periinfarct areas after MCAO. In the cultured pericytes, PDGF-B induced cell growth and anti-apoptotic responses through Akt. Furthermore, PDGF-B significantly increased the expression of nerve growth factor (NGF) and neurotrophin-3 (NT-3) through Akt in the pericytes. Thus, the PDGFRβ-Akt signaling in brain pericytes may play various important roles leading to neuroprotection after ischemic stroke.

Original languageEnglish
Pages (from-to)1-9
Number of pages9
JournalCurrent Neurovascular Research
Volume9
Issue number1
DOIs
Publication statusPublished - Jan 1 2012

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Platelet-Derived Growth Factor Receptors
Pericytes
Proto-Oncogene Proteins c-sis
Brain Injuries
Brain
Middle Cerebral Artery Infarction
Stroke
Neurotrophin 3
Nerve Growth Factor
Astrocytes
Endothelial Cells
Phosphorylation
Neurons

All Science Journal Classification (ASJC) codes

  • Neurology
  • Developmental Neuroscience
  • Cellular and Molecular Neuroscience

Cite this

PDGF receptor β signaling in pericytes following ischemic brain injury. / Arimura, Koichi; Ago, Tetsuro; Kamouchi, Masahiro; Nakamura, Kuniyuki; Ishitsuka, Koji; Kuroda, Junya; Sugimori, Hiroshi; Ooboshi, Hiroaki; Sasaki, Tomio; Kitazono, Takanari.

In: Current Neurovascular Research, Vol. 9, No. 1, 01.01.2012, p. 1-9.

Research output: Contribution to journalArticle

Arimura, Koichi ; Ago, Tetsuro ; Kamouchi, Masahiro ; Nakamura, Kuniyuki ; Ishitsuka, Koji ; Kuroda, Junya ; Sugimori, Hiroshi ; Ooboshi, Hiroaki ; Sasaki, Tomio ; Kitazono, Takanari. / PDGF receptor β signaling in pericytes following ischemic brain injury. In: Current Neurovascular Research. 2012 ; Vol. 9, No. 1. pp. 1-9.
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