PDGFs regulate tooth germ proliferation and ameloblast differentiation

Nan Wu, Tsutomu Iwamoto, Yu Sugawara, Masaharu Futaki, Keigo Yoshizaki, Shinya Yamamoto, Aya Yamada, Takashi Nakamura, Kazuaki Nonaka, Satoshi Fukumoto

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Objective: The purpose of this study was to elucidate the effects of platelet-derived growth factors (PDGFs) during tooth development, as well as the mechanisms underlying the interactions of growth factors with PDGF signalling during odontogenesis. Design: We used an ex vivo tooth germ organ culture system and two dental cell lines, SF2 cells and mDP cells, as models of odontogenesis. AG17, a tyrosine kinase inhibitor, was utilised for blocking PDGF receptor signalling. To analyse the expressions of PDGFs, reverse transcriptase (RT)-PCR and immunohistochemistry were performed. Proliferation was examined using a BrdU incorporation assay for the organ cultures and a cell counting kit for the cell lines. The expressions of Fgf2 and ameloblastin were analysed by real-time RT-PCR. Results: The PDGF ligands PDGF-A and PDGF-B, and their receptors, PDGFRα and PDGFRβ, were expressed throughout the initial stages of tooth development. In the tooth germ organ cultures, PDGF-AA, but not PDGF-BB, accelerated cusp formation. Conversely, AG17 suppressed both growth and cusp formation of tooth germs. Exogenous PDGF-BB promoted mDP cell proliferation. Furthermore, PDGF-AA decreased Fgf2 expression and increased that of ameloblastin, a marker of differentiated ameloblasts. Conclusion: Our results indicate that PDGFs are involved in initial tooth development and regulate tooth size and shape, as well as ameloblast differentiation.

Original languageEnglish
Pages (from-to)426-434
Number of pages9
JournalArchives of Oral Biology
Volume55
Issue number6
DOIs
Publication statusPublished - Jun 1 2010

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Tooth Germ
Ameloblasts
Platelet-Derived Growth Factor
Tooth
Organ Culture Techniques
Odontogenesis
Reverse Transcriptase Polymerase Chain Reaction
Proto-Oncogene Proteins c-sis
Platelet-Derived Growth Factor Receptors
Cell Line
Bromodeoxyuridine
Protein-Tyrosine Kinases
Real-Time Polymerase Chain Reaction
Intercellular Signaling Peptides and Proteins
Immunohistochemistry
Cell Proliferation
Ligands
Growth

All Science Journal Classification (ASJC) codes

  • Otorhinolaryngology
  • Dentistry(all)
  • Cell Biology

Cite this

PDGFs regulate tooth germ proliferation and ameloblast differentiation. / Wu, Nan; Iwamoto, Tsutomu; Sugawara, Yu; Futaki, Masaharu; Yoshizaki, Keigo; Yamamoto, Shinya; Yamada, Aya; Nakamura, Takashi; Nonaka, Kazuaki; Fukumoto, Satoshi.

In: Archives of Oral Biology, Vol. 55, No. 6, 01.06.2010, p. 426-434.

Research output: Contribution to journalArticle

Wu, N, Iwamoto, T, Sugawara, Y, Futaki, M, Yoshizaki, K, Yamamoto, S, Yamada, A, Nakamura, T, Nonaka, K & Fukumoto, S 2010, 'PDGFs regulate tooth germ proliferation and ameloblast differentiation', Archives of Oral Biology, vol. 55, no. 6, pp. 426-434. https://doi.org/10.1016/j.archoralbio.2010.03.011
Wu, Nan ; Iwamoto, Tsutomu ; Sugawara, Yu ; Futaki, Masaharu ; Yoshizaki, Keigo ; Yamamoto, Shinya ; Yamada, Aya ; Nakamura, Takashi ; Nonaka, Kazuaki ; Fukumoto, Satoshi. / PDGFs regulate tooth germ proliferation and ameloblast differentiation. In: Archives of Oral Biology. 2010 ; Vol. 55, No. 6. pp. 426-434.
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