Pdms-anchored nanowires for high throughput micro-RNA extraction from extracellular vesicles in body fluid

Daiki Takeshita, Takao Yasui, He Yong, Takeshi Yanagida, Noritada Kaji, Masaki Kanai, Kazuki Nagashima, Tomoji Kawai, Yoshinobu Baba

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We anchored zinc oxide nanowires (ZnO) in PDMS microchannel for high throughput capture of extracellular vesicles and microRNA extraction in them from body fluids. Using this device, we achieved vesicles capture from urine sample within 10 min, which is faster than using the conventional methods, such as precipitation kit or ultracentrifugation. And we demonstrated microRNA extraction from the captured vesicles. These results highlighted that we realized high throughput microRNA extraction from body fluid.

Original languageEnglish
Title of host publicationMicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences
PublisherChemical and Biological Microsystems Society
Pages1516-1518
Number of pages3
ISBN (Electronic)9780979806483
Publication statusPublished - Jan 1 2015
Event19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015 - Gyeongju, Korea, Republic of
Duration: Oct 25 2015Oct 29 2015

Other

Other19th International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2015
CountryKorea, Republic of
CityGyeongju
Period10/25/1510/29/15

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All Science Journal Classification (ASJC) codes

  • Control and Systems Engineering

Cite this

Takeshita, D., Yasui, T., Yong, H., Yanagida, T., Kaji, N., Kanai, M., ... Baba, Y. (2015). Pdms-anchored nanowires for high throughput micro-RNA extraction from extracellular vesicles in body fluid. In MicroTAS 2015 - 19th International Conference on Miniaturized Systems for Chemistry and Life Sciences (pp. 1516-1518). Chemical and Biological Microsystems Society.