Pediococcus lolii sp. nov., isolated from ryegrass silage

Katsumi Doi, Yousuke Nishizaki, Yasuhiro Fujino, Toshihisa Ohshima, Sadahiro Ohmomo, Seiya Ogata

Research output: Contribution to journalArticle

17 Citations (Scopus)

Abstract

A Gram-positive, coccus-shaped, lactic acid bacterium, strain NGRI 0510QT, was isolated from ryegrass silage produced in Okinawa Prefecture, Japan. The cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. The strain is homofermentative and produces D- and L-lactic acid from glucose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGRI 0510QT belongs to the genus Pediococcus and clusters within the Pediococcus acidilactici and Pediococcus pentosaceus group, with 98.2 and 96.9% sequence identity, respectively. DNA-DNA relatedness between strain NGRI 0510QT and P. acidilactici JCM 8797T and P. pentosaceus JCM 5890T was 19.3 and 17.3 %, respectively. Based on its phenotypic characteristics, phylogenetic relationship and DNA-DNA relatedness, NGRI 0510QT (=JCM 15055T=DSM 19927T) represents the type strain of a novel species, for which the name Pediococcus lolii sp. nov. is proposed.

Original languageEnglish
Pages (from-to)1007-1010
Number of pages4
JournalInternational Journal of Systematic and Evolutionary Microbiology
Volume59
Issue number5
DOIs
Publication statusPublished - Nov 10 2009

Fingerprint

Pediococcus
Lolium
Silage
silage
grass silage
DNA
Lactic Acid
new species
Pediococcus acidilactici
Pediococcus pentosaceus
relatedness
phylogenetics
Gram-Positive Cocci
acid
rRNA Genes
Coccus
Ryukyu Archipelago
Names
phylogeny
Japan

All Science Journal Classification (ASJC) codes

  • Microbiology
  • Ecology, Evolution, Behavior and Systematics

Cite this

Pediococcus lolii sp. nov., isolated from ryegrass silage. / Doi, Katsumi; Nishizaki, Yousuke; Fujino, Yasuhiro; Ohshima, Toshihisa; Ohmomo, Sadahiro; Ogata, Seiya.

In: International Journal of Systematic and Evolutionary Microbiology, Vol. 59, No. 5, 10.11.2009, p. 1007-1010.

Research output: Contribution to journalArticle

Doi, Katsumi ; Nishizaki, Yousuke ; Fujino, Yasuhiro ; Ohshima, Toshihisa ; Ohmomo, Sadahiro ; Ogata, Seiya. / Pediococcus lolii sp. nov., isolated from ryegrass silage. In: International Journal of Systematic and Evolutionary Microbiology. 2009 ; Vol. 59, No. 5. pp. 1007-1010.
@article{a13769db94c34414be332d0867db3f7e,
title = "Pediococcus lolii sp. nov., isolated from ryegrass silage",
abstract = "A Gram-positive, coccus-shaped, lactic acid bacterium, strain NGRI 0510QT, was isolated from ryegrass silage produced in Okinawa Prefecture, Japan. The cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. The strain is homofermentative and produces D- and L-lactic acid from glucose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGRI 0510QT belongs to the genus Pediococcus and clusters within the Pediococcus acidilactici and Pediococcus pentosaceus group, with 98.2 and 96.9{\%} sequence identity, respectively. DNA-DNA relatedness between strain NGRI 0510QT and P. acidilactici JCM 8797T and P. pentosaceus JCM 5890T was 19.3 and 17.3 {\%}, respectively. Based on its phenotypic characteristics, phylogenetic relationship and DNA-DNA relatedness, NGRI 0510QT (=JCM 15055T=DSM 19927T) represents the type strain of a novel species, for which the name Pediococcus lolii sp. nov. is proposed.",
author = "Katsumi Doi and Yousuke Nishizaki and Yasuhiro Fujino and Toshihisa Ohshima and Sadahiro Ohmomo and Seiya Ogata",
year = "2009",
month = "11",
day = "10",
doi = "10.1099/ijs.0.005793-0",
language = "English",
volume = "59",
pages = "1007--1010",
journal = "International Journal of Systematic and Evolutionary Microbiology",
issn = "1466-5026",
publisher = "Society for General Microbiology",
number = "5",

}

TY - JOUR

T1 - Pediococcus lolii sp. nov., isolated from ryegrass silage

AU - Doi, Katsumi

AU - Nishizaki, Yousuke

AU - Fujino, Yasuhiro

AU - Ohshima, Toshihisa

AU - Ohmomo, Sadahiro

AU - Ogata, Seiya

PY - 2009/11/10

Y1 - 2009/11/10

N2 - A Gram-positive, coccus-shaped, lactic acid bacterium, strain NGRI 0510QT, was isolated from ryegrass silage produced in Okinawa Prefecture, Japan. The cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. The strain is homofermentative and produces D- and L-lactic acid from glucose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGRI 0510QT belongs to the genus Pediococcus and clusters within the Pediococcus acidilactici and Pediococcus pentosaceus group, with 98.2 and 96.9% sequence identity, respectively. DNA-DNA relatedness between strain NGRI 0510QT and P. acidilactici JCM 8797T and P. pentosaceus JCM 5890T was 19.3 and 17.3 %, respectively. Based on its phenotypic characteristics, phylogenetic relationship and DNA-DNA relatedness, NGRI 0510QT (=JCM 15055T=DSM 19927T) represents the type strain of a novel species, for which the name Pediococcus lolii sp. nov. is proposed.

AB - A Gram-positive, coccus-shaped, lactic acid bacterium, strain NGRI 0510QT, was isolated from ryegrass silage produced in Okinawa Prefecture, Japan. The cell is non-spore-forming, non-motile, and occurs in pairs or tetrads. The strain is homofermentative and produces D- and L-lactic acid from glucose. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain NGRI 0510QT belongs to the genus Pediococcus and clusters within the Pediococcus acidilactici and Pediococcus pentosaceus group, with 98.2 and 96.9% sequence identity, respectively. DNA-DNA relatedness between strain NGRI 0510QT and P. acidilactici JCM 8797T and P. pentosaceus JCM 5890T was 19.3 and 17.3 %, respectively. Based on its phenotypic characteristics, phylogenetic relationship and DNA-DNA relatedness, NGRI 0510QT (=JCM 15055T=DSM 19927T) represents the type strain of a novel species, for which the name Pediococcus lolii sp. nov. is proposed.

UR - http://www.scopus.com/inward/record.url?scp=67649744186&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=67649744186&partnerID=8YFLogxK

U2 - 10.1099/ijs.0.005793-0

DO - 10.1099/ijs.0.005793-0

M3 - Article

VL - 59

SP - 1007

EP - 1010

JO - International Journal of Systematic and Evolutionary Microbiology

JF - International Journal of Systematic and Evolutionary Microbiology

SN - 1466-5026

IS - 5

ER -