Bacteriocin ISK-1 is a proteinaceous inhibitory substance produced by Pediococcus sp. ISK-1 isolated from well-aged Nukadoko (Kimura et al., 1997a). Bacteriocin ISK-1 was purified by acid treatment, ammonium sulfate precipitation, cation-exchange chromatography and reversed-phase high performance chromatography from the culture supernatant of Pediococcus sp. ISK-1. Purification of bacteriocin ISK-1 resulted in 30-fold increase in the specific activity and the recovery was 17%. Molecular mass of bacteriocin ISK-1 determined by fast atom bombardment-mass spectrometry was 2,960. The amino acid composition analysis of bacteriocin ISK-1 revealed that it contained lanthionine and/or 3-methyllanthionine which is a characteristic of lantibiotics. The N-terminal amino acid sequence analysis indicated the first seven N-terminal amino acid residues as, NH2-K-K-K-S-G-V-I. The primary sequence showed significant homology to the lantibiotics lacticin 481 from Lactococcus lactis and variacin from Micrococcus varians, which suggests that bacteriocin ISK-1 is a novel lantibiotic belonging to a lacticin-481 type.
|Number of pages||13|
|Journal||Journal of the Faculty of Agriculture, Kyushu University|
|Publication status||Published - Nov 1 1998|
All Science Journal Classification (ASJC) codes
- Agronomy and Crop Science