Pediococcus sp. ISK-1 Produces a Novel Lantibiotic, Bacteriocin ISK-1

Hirokazu Kimura, Hiromi Matsusaki, Toshihiro Sashihara, Kenji Sonomoto, Ayaaki Ishizaki

Research output: Contribution to journalArticle

Abstract

Bacteriocin ISK-1 is a proteinaceous inhibitory substance produced by Pediococcus sp. ISK-1 isolated from well-aged Nukadoko (Kimura et al., 1997a). Bacteriocin ISK-1 was purified by acid treatment, ammonium sulfate precipitation, cation-exchange chromatography and reversed-phase high performance chromatography from the culture supernatant of Pediococcus sp. ISK-1. Purification of bacteriocin ISK-1 resulted in 30-fold increase in the specific activity and the recovery was 17%. Molecular mass of bacteriocin ISK-1 determined by fast atom bombardment-mass spectrometry was 2,960. The amino acid composition analysis of bacteriocin ISK-1 revealed that it contained lanthionine and/or 3-methyllanthionine which is a characteristic of lantibiotics. The N-terminal amino acid sequence analysis indicated the first seven N-terminal amino acid residues as, NH2-K-K-K-S-G-V-I. The primary sequence showed significant homology to the lantibiotics lacticin 481 from Lactococcus lactis and variacin from Micrococcus varians, which suggests that bacteriocin ISK-1 is a novel lantibiotic belonging to a lacticin-481 type.

Original languageEnglish
Pages (from-to)195-207
Number of pages13
JournalJournal of the Faculty of Agriculture, Kyushu University
Volume43
Issue number1-2
Publication statusPublished - Nov 1 1998

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Pediococcus
Bacteriocins
bacteriocins
Kocuria varians
Fast Atom Bombardment Mass Spectrometry
Amino Acids
Micrococcus
Lactococcus lactis
Protein Sequence Analysis
Ammonium Sulfate
Reverse-Phase Chromatography
acid treatment
amino acid composition
ammonium sulfate
Cations
Chromatography
chromatography
amino acid sequences
sequence analysis
mass spectrometry

All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Agronomy and Crop Science

Cite this

Kimura, H., Matsusaki, H., Sashihara, T., Sonomoto, K., & Ishizaki, A. (1998). Pediococcus sp. ISK-1 Produces a Novel Lantibiotic, Bacteriocin ISK-1. Journal of the Faculty of Agriculture, Kyushu University, 43(1-2), 195-207.

Pediococcus sp. ISK-1 Produces a Novel Lantibiotic, Bacteriocin ISK-1. / Kimura, Hirokazu; Matsusaki, Hiromi; Sashihara, Toshihiro; Sonomoto, Kenji; Ishizaki, Ayaaki.

In: Journal of the Faculty of Agriculture, Kyushu University, Vol. 43, No. 1-2, 01.11.1998, p. 195-207.

Research output: Contribution to journalArticle

Kimura, H, Matsusaki, H, Sashihara, T, Sonomoto, K & Ishizaki, A 1998, 'Pediococcus sp. ISK-1 Produces a Novel Lantibiotic, Bacteriocin ISK-1', Journal of the Faculty of Agriculture, Kyushu University, vol. 43, no. 1-2, pp. 195-207.
Kimura H, Matsusaki H, Sashihara T, Sonomoto K, Ishizaki A. Pediococcus sp. ISK-1 Produces a Novel Lantibiotic, Bacteriocin ISK-1. Journal of the Faculty of Agriculture, Kyushu University. 1998 Nov 1;43(1-2):195-207.
Kimura, Hirokazu ; Matsusaki, Hiromi ; Sashihara, Toshihiro ; Sonomoto, Kenji ; Ishizaki, Ayaaki. / Pediococcus sp. ISK-1 Produces a Novel Lantibiotic, Bacteriocin ISK-1. In: Journal of the Faculty of Agriculture, Kyushu University. 1998 ; Vol. 43, No. 1-2. pp. 195-207.
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