Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner

Yasutomi Kamei, Takayoshi Suganami, Takashi Kohda, Fumitoshi Ishino, Kazuki Yasuda, Shinji Miura, Osamu Ezaki, Yoshihiro Ogawa

Research output: Contribution to journalArticle

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Abstract

Paternally expressed 1 (Peg1)/mesoderm specific transcript (Mest) is an imprinted gene, which is only transcribed from the paternal (father's) allele. In some human cancer tissues, an alternatively spliced variant of PEG1/MEST mRNA using a different promoter of a distinct first exon is expressed from both paternal and maternal alleles. We previously reported that Peg1/Mest expression was markedly up-regulated in obese adipose tissue in mice. Moreover, transgenic overexpression of Peg1/Mest in the adipose tissue resulted in the enlargement of adipocytes in size. Given the potential pathophysiologic relevance in obesity, we examined the nature of increased expression of Peg1/Mest in obese adipose tissue. In obese adipose tissue, expression of Peg1/Mest was increased, but not that of other imprinted genes tested. The transcription rate of Peg1/Mest was increased in obese adipose tissue. We found at least four isoforms of mouse Peg1/Mest generated by use of the alternative first exons. We also demonstrated that the abundantly expressed Peg1/Mest in obese adipose tissue retained monoallelic expression. This is the first report of monoallelic induction of Peg1/Mest in adult tissues.

Original languageEnglish
Pages (from-to)91-96
Number of pages6
JournalFEBS Letters
Volume581
Issue number1
DOIs
Publication statusPublished - Jan 9 2007

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Mesoderm
Adipose Tissue
Protein Isoforms
Alleles
Tissue
Exons
Genes
Transcription
Adipocytes
Fathers
Obesity
Mothers
Messenger RNA

All Science Journal Classification (ASJC) codes

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner. / Kamei, Yasutomi; Suganami, Takayoshi; Kohda, Takashi; Ishino, Fumitoshi; Yasuda, Kazuki; Miura, Shinji; Ezaki, Osamu; Ogawa, Yoshihiro.

In: FEBS Letters, Vol. 581, No. 1, 09.01.2007, p. 91-96.

Research output: Contribution to journalArticle

Kamei, Y, Suganami, T, Kohda, T, Ishino, F, Yasuda, K, Miura, S, Ezaki, O & Ogawa, Y 2007, 'Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner', FEBS Letters, vol. 581, no. 1, pp. 91-96. https://doi.org/10.1016/j.febslet.2006.12.002
Kamei, Yasutomi ; Suganami, Takayoshi ; Kohda, Takashi ; Ishino, Fumitoshi ; Yasuda, Kazuki ; Miura, Shinji ; Ezaki, Osamu ; Ogawa, Yoshihiro. / Peg1/Mest in obese adipose tissue is expressed from the paternal allele in an isoform-specific manner. In: FEBS Letters. 2007 ; Vol. 581, No. 1. pp. 91-96.
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