TY - JOUR
T1 - Penicillin-binding protein sensitive to cephalexin in sporulation of Bacillus cereus
AU - Miyamoto, Takahisa
AU - Yamaguchi, Ken Ichiro
AU - Sayed, Md Abu
AU - Sasahara, Ryo
AU - Honjoh, Ken-ichi
AU - Hatano, Shoji
PY - 1997/1/1
Y1 - 1997/1/1
N2 - Cephalexin, cefaclor, cefadroxil, and cefotaxime strongly inhibited sporulation of Bacillus cereus ts-4 at 1 μg/ml. Cephalexin was most inhibitory on the sporulation of B. cereus when the antibiotic was added at 3 h after induction of sporulation by nutrient downshift technique. Examination of 4',6-diamidino-2-phenylindole-stained cells by fluorescence-phase contrast microscopy showed that cephalexin inhibited the formation of asymmetric septum. By using [3H]penicillin, eight penicillin-binding proteins (PBPs) were detected from the cells of B. cereus ts-4. Among them, four PBPs were also detected in sporulating cells. Affinity of cephalexin to PBPs were measured indirectly by competition for subsequent binding of radioactive penicillin G. Cephalexin strongly bound to PBP 4 with molecular weight of 72,000 in sporulating cells.
AB - Cephalexin, cefaclor, cefadroxil, and cefotaxime strongly inhibited sporulation of Bacillus cereus ts-4 at 1 μg/ml. Cephalexin was most inhibitory on the sporulation of B. cereus when the antibiotic was added at 3 h after induction of sporulation by nutrient downshift technique. Examination of 4',6-diamidino-2-phenylindole-stained cells by fluorescence-phase contrast microscopy showed that cephalexin inhibited the formation of asymmetric septum. By using [3H]penicillin, eight penicillin-binding proteins (PBPs) were detected from the cells of B. cereus ts-4. Among them, four PBPs were also detected in sporulating cells. Affinity of cephalexin to PBPs were measured indirectly by competition for subsequent binding of radioactive penicillin G. Cephalexin strongly bound to PBP 4 with molecular weight of 72,000 in sporulating cells.
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U2 - 10.1016/S0944-5013(97)80032-8
DO - 10.1016/S0944-5013(97)80032-8
M3 - Article
C2 - 9352657
AN - SCOPUS:0031238849
VL - 152
SP - 227
EP - 232
JO - Microbiological Research
JF - Microbiological Research
SN - 0944-5013
IS - 3
ER -