Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts

Kenji Okuda, Anne Laure Chateigner-Boutin, Takahiro Nakamura, Etienne Delannoy, Mamoru Sugita, Fumiyoshi Myouga, Reiko Motohashi, Kazuo Shinozaki, Ian Small, Toshiharu Shikanai

Research output: Contribution to journalArticle

164 Citations (Scopus)

Abstract

The plant-specific DYW subclass of pentatricopeptide repeat proteins has been postulated to be involved in RNA editing of organelle transcripts. We discovered that the DYW proteins CHLORORESPIRATORY REDUCTION22 (CRR22) and CRR28 are required for editing of multiple plastid transcripts but that their DYW motifs are dispensable for editing activity in vivo. Replacement of the DYW motifs of CRR22 and CRR28 by that of CRR2, which has been shown to be capable of endonucleolytic cleavage, blocks the editing activity of both proteins. In return, the DYW motifs of neither CRR22 nor CRR28 can functionally replace that of CRR2. We propose that different DYW family members have acquired distinct functions in the divergent processes of RNA maturation, including RNA cleavage and RNA editing.

Original languageEnglish
Pages (from-to)146-156
Number of pages11
JournalPlant Cell
Volume21
Issue number1
DOIs
Publication statusPublished - Jan 1 2009

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RNA Cleavage
RNA Editing
RNA editing
Chloroplasts
Arabidopsis
chloroplasts
RNA
Proteins
Plastids
proteins
Organelles
plastids
organelles

All Science Journal Classification (ASJC) codes

  • Plant Science
  • Cell Biology

Cite this

Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts. / Okuda, Kenji; Chateigner-Boutin, Anne Laure; Nakamura, Takahiro; Delannoy, Etienne; Sugita, Mamoru; Myouga, Fumiyoshi; Motohashi, Reiko; Shinozaki, Kazuo; Small, Ian; Shikanai, Toshiharu.

In: Plant Cell, Vol. 21, No. 1, 01.01.2009, p. 146-156.

Research output: Contribution to journalArticle

Okuda, K, Chateigner-Boutin, AL, Nakamura, T, Delannoy, E, Sugita, M, Myouga, F, Motohashi, R, Shinozaki, K, Small, I & Shikanai, T 2009, 'Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts', Plant Cell, vol. 21, no. 1, pp. 146-156. https://doi.org/10.1105/tpc.108.064667
Okuda, Kenji ; Chateigner-Boutin, Anne Laure ; Nakamura, Takahiro ; Delannoy, Etienne ; Sugita, Mamoru ; Myouga, Fumiyoshi ; Motohashi, Reiko ; Shinozaki, Kazuo ; Small, Ian ; Shikanai, Toshiharu. / Pentatricopeptide repeat proteins with the DYW motif have distinct molecular functions in RNA editing and RNA cleavage in Arabidopsis chloroplasts. In: Plant Cell. 2009 ; Vol. 21, No. 1. pp. 146-156.
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