Peptide array for the comprehensive analysis of intracellular signals aiming to drug screening

Yoshiki Katayama, Tatsuhiko Sonoda, Yusuke Inoue, Takayuki Yamaji, Aishan Han, Kazuki Inamori, Osamu Okitsu, Masaharu Murata, Takuro Niidome

Research output: Contribution to conferencePaper

Abstract

New peptide array, on which many substrate peptides for protein kinases are immobilized, was developed. If the array was treated with isolated protein kinases or cell lysate, each peptide was phosphorylated dependent on their properties, after the optimized immobilization and masking procedures. Phosphorylation in each spot was detected by fluorescence on glass slide or 2 dimensional SPR on gold chip using anti-phospho antibody. In the glass array using Cy-3 labeled antibody for the detection, drug-specific profiles of peptide phosphorylation were obtained. Using this method, new assay system for the drug screening or fuctional analysis of unknown gene may be possible.

Original languageEnglish
Pages4849-4850
Number of pages2
Publication statusPublished - 2005
Event54th SPSJ Symposium on Macromolecules - Yamagata, Japan
Duration: Sep 20 2005Sep 22 2005

Other

Other54th SPSJ Symposium on Macromolecules
CountryJapan
CityYamagata
Period9/20/059/22/05

All Science Journal Classification (ASJC) codes

  • Engineering(all)

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  • Cite this

    Katayama, Y., Sonoda, T., Inoue, Y., Yamaji, T., Han, A., Inamori, K., Okitsu, O., Murata, M., & Niidome, T. (2005). Peptide array for the comprehensive analysis of intracellular signals aiming to drug screening. 4849-4850. Paper presented at 54th SPSJ Symposium on Macromolecules, Yamagata, Japan.