Peptides binding to a Gb3 mimic selected from a phage library

Yoshiko Miura, Yuuki Sasao, Masamichi Kamihira, Akio Sakaki, Shinji Iijima, Kazukiyo Kobayashi

Research output: Contribution to journalArticlepeer-review

23 Citations (Scopus)

Abstract

Peptides binding to a Gb3 mimic were selected from 12-mer peptide library. The self-assembled monolayer (SAM) of a Gb3 mimic was formed on the gold surface, and biopanning was carried out with the phage display peptide library. After three rounds of biopanning, four individual sequences were obtained from 10 phage clones, and the selected peptides having the specific 7-mer sequence (FHENWPS) showed affinities to the Gb3 mimic as strong as to RCA120. Molecular dynamics calculations suggested that the peptides bound to the Gb3 mimic by hydrophobic interaction and hydrogen bonding formation, and the cooperative interactions played an important role in the recognition. The Stx-1 binding was inhibited by the peptides.

Original languageEnglish
Pages (from-to)131-138
Number of pages8
JournalBiochimica et Biophysica Acta - General Subjects
Volume1673
Issue number3
DOIs
Publication statusPublished - Aug 4 2004
Externally publishedYes

All Science Journal Classification (ASJC) codes

  • Biophysics
  • Biochemistry
  • Molecular Biology

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