Peptidoglycan activation of the proPO-system without a peptidoglycan receptor protein (PGRP)?

Haipeng Liu, Chenglin Wu, Yasuyuki Matsuda, Shun ichiro Kawabata, Bok Luel Lee, Kenneth Söderhäll, Irene Söderhäll

Research output: Contribution to journalArticle

30 Citations (Scopus)

Abstract

Recognition of microbial polysaccharide by pattern recognition receptors triggers the prophenoloxidase (proPO) cascade, resulting in melanin synthesis and its deposition on the surface of invading pathogens. Several masquerade-like proteins and serine proteinase homologues have been shown to be involved in the proPO activation in insects. In this study, a novel serine proteinase homologue, Pl-SPH2, was found and isolated as a 30 kDa protein from hemocytes of the freshwater crayfish, Pacifastacus leniusculus, by its binding property to a partially lysozyme digested or TCA-treated insoluble Lysine (Lys)-type peptidoglycan (PGN) and soluble polymeric Lys-type PGN. Two other proteins, the Pl-SPH1 and lipopolysaccharide- and β-1,3-glucan-binding protein (LGBP) were also found in the several different PGN-binding assays. However no PGRP homologue was detected. Neither was any putative PGRP found after searching available crustacean sequence databases. If RNA interference of Pl-SPH2, Pl-SPH1 or LGBP in the crayfish hematopoietic tissue cell culture was performed, it resulted in lower PO activity following activation of the proPO-system by soluble Lys-type PGN. Taken together, we report for the first time that Lys-type PGN is a trigger of proPO-system activation in a crustacean and that two Pl-SPHs are involved in this activation possibly by forming a complex with LGBP and without a PGRP.

Original languageEnglish
Pages (from-to)51-61
Number of pages11
JournalDevelopmental and Comparative Immunology
Volume35
Issue number1
DOIs
Publication statusPublished - Jan 1 2011

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Peptidoglycan
Lysine
Lipopolysaccharides
Astacoidea
Proteins
Serine Proteases
Pattern Recognition Receptors
Hemocytes
Melanins
Muramidase
RNA Interference
Fresh Water
Polysaccharides
Insects
pro-phenoloxidase
peptidoglycan receptor
Cell Culture Techniques
Databases
glucan-binding proteins

All Science Journal Classification (ASJC) codes

  • Immunology
  • Developmental Biology

Cite this

Peptidoglycan activation of the proPO-system without a peptidoglycan receptor protein (PGRP)? / Liu, Haipeng; Wu, Chenglin; Matsuda, Yasuyuki; Kawabata, Shun ichiro; Lee, Bok Luel; Söderhäll, Kenneth; Söderhäll, Irene.

In: Developmental and Comparative Immunology, Vol. 35, No. 1, 01.01.2011, p. 51-61.

Research output: Contribution to journalArticle

Liu, Haipeng ; Wu, Chenglin ; Matsuda, Yasuyuki ; Kawabata, Shun ichiro ; Lee, Bok Luel ; Söderhäll, Kenneth ; Söderhäll, Irene. / Peptidoglycan activation of the proPO-system without a peptidoglycan receptor protein (PGRP)?. In: Developmental and Comparative Immunology. 2011 ; Vol. 35, No. 1. pp. 51-61.
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