Peptidoglycan and lipopolysaccharide activate PLCγ2, leading to enhanced cytokine production in macrophages and dendritic cells

Daisuke Aki, Yasumasa Minoda, Hideyuki Yoshida, Satoko Watanabe, Ryoko Yoshida, Giichi Takaesu, Takatoshi Chinen, Toshiya Inaba, Masaki Hikida, Tomohiro Kurosaki, Kazuko Saeki, Akihiko Yoshimura

Research output: Contribution to journalArticle

51 Citations (Scopus)


In macrophages and monocytes, microbial components trigger the production of pro-inflammatory cytokine through Toll-like receptors (TLRs). Although major TLR signaling pathways are mediated by serine/ threonine kinases, including TAK1, IKK and MAP kinases, tyrosine phosphorylation of intracellular proteins by TLR ligands has been suggested in a number of reports. Here, we demonstrated that peptidoglycan (PGN) of a Gram-positive bacterial cell wall component, a TLR2 ligand and lipopoysaccharide (LPS) of a Gram-positive bacterial component, a TLR4 ligand induced tyrosine phosphorylation of phospholipase Cγ-2 (PLCγ2), leading to intracellular free Ca2+ mobilization in bone marrow-derived macrophages (BMMφ) and bone marrow-derived dendritic cells (BMDC). PGN- and LPS-induced Ca2+ mobilization was not observed in BMDC from PLCγ2 knockout mice. Thus, PLCγ2 is essential for TLR2 and TLR4-mediated Ca2+ flux. In PLCγ2-knockdown cells, PGN-induced IκB-α phosphorylation and p38 activation were reduced. Moreover, PLCγ2 was necessary for the full production of TNF-α and IL-6. These data indicate that the PLCγ2 pathway plays an important role in bacterial ligands-induced activation of macrophages and dendritic cells. Journal compilation

Original languageEnglish
Pages (from-to)199-208
Number of pages10
JournalGenes to Cells
Issue number2
Publication statusPublished - Feb 1 2008


All Science Journal Classification (ASJC) codes

  • Genetics
  • Cell Biology

Cite this