Performance evaluation and comparison of four SPR immunoassays for rapid and label-free detection of TNT

Dhesingh Ravi Shankaran, Kiyoshi Matsumoto, Kiyoshi Toko, Norio Miura

Research output: Contribution to journalArticle

13 Citations (Scopus)


A simple and highly sensitive surface-plasmon-resonance (SPR) immunoassay has been demonstrated for rapid and label-free detection of 2,4,6- trinitrotoluene (TNT). The immunoreaction between a home-made polyclonal anti-2,4,6-trinitrophenyl-keyhole-limpet-hemocyanine antibody (P-TNPh-KLH Ab) with a physically immobilized TNPh-ovalbumin (TNPh-OVA) conjugate was utilized for TNT quantification based on the principle of indirect competitive immunoreaction. The performance of the P-TNPh-KLH Ab was compared with three commercially-available antibodies and was observed that the home-made antibody exhibited the highest sensitivity to TNT (1 ppt) among the other antibodies with a response time of 2 min.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
Issue number2
Publication statusPublished - Jan 1 2006


All Science Journal Classification (ASJC) codes

  • Electrochemistry

Cite this