Performance evaluation and comparison of four SPR immunoassays for rapid and label-free detection of TNT

Dhesingh Ravi Shankaran, Kiyoshi Matsumoto, Kiyoshi Toko, Norio Miura

Research output: Contribution to journalArticle

13 Citations (Scopus)

Abstract

A simple and highly sensitive surface-plasmon-resonance (SPR) immunoassay has been demonstrated for rapid and label-free detection of 2,4,6- trinitrotoluene (TNT). The immunoreaction between a home-made polyclonal anti-2,4,6-trinitrophenyl-keyhole-limpet-hemocyanine antibody (P-TNPh-KLH Ab) with a physically immobilized TNPh-ovalbumin (TNPh-OVA) conjugate was utilized for TNT quantification based on the principle of indirect competitive immunoreaction. The performance of the P-TNPh-KLH Ab was compared with three commercially-available antibodies and was observed that the home-made antibody exhibited the highest sensitivity to TNT (1 ppt) among the other antibodies with a response time of 2 min.

Original languageEnglish
Pages (from-to)141-144
Number of pages4
JournalElectrochemistry
Volume74
Issue number2
DOIs
Publication statusPublished - Jan 1 2006

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Trinitrotoluene
Surface plasmon resonance
Antibodies
Labels
Ovalbumin

All Science Journal Classification (ASJC) codes

  • Electrochemistry

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Performance evaluation and comparison of four SPR immunoassays for rapid and label-free detection of TNT. / Ravi Shankaran, Dhesingh; Matsumoto, Kiyoshi; Toko, Kiyoshi; Miura, Norio.

In: Electrochemistry, Vol. 74, No. 2, 01.01.2006, p. 141-144.

Research output: Contribution to journalArticle

Ravi Shankaran, Dhesingh ; Matsumoto, Kiyoshi ; Toko, Kiyoshi ; Miura, Norio. / Performance evaluation and comparison of four SPR immunoassays for rapid and label-free detection of TNT. In: Electrochemistry. 2006 ; Vol. 74, No. 2. pp. 141-144.
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