TY - JOUR
T1 - Performance evaluation and comparison of four SPR immunoassays for rapid and label-free detection of TNT
AU - Ravi Shankaran, Dhesingh
AU - Matsumoto, Kiyoshi
AU - Toko, Kiyoshi
AU - Miura, Norio
PY - 2006
Y1 - 2006
N2 - A simple and highly sensitive surface-plasmon-resonance (SPR) immunoassay has been demonstrated for rapid and label-free detection of 2,4,6- trinitrotoluene (TNT). The immunoreaction between a home-made polyclonal anti-2,4,6-trinitrophenyl-keyhole-limpet-hemocyanine antibody (P-TNPh-KLH Ab) with a physically immobilized TNPh-ovalbumin (TNPh-OVA) conjugate was utilized for TNT quantification based on the principle of indirect competitive immunoreaction. The performance of the P-TNPh-KLH Ab was compared with three commercially-available antibodies and was observed that the home-made antibody exhibited the highest sensitivity to TNT (1 ppt) among the other antibodies with a response time of 2 min.
AB - A simple and highly sensitive surface-plasmon-resonance (SPR) immunoassay has been demonstrated for rapid and label-free detection of 2,4,6- trinitrotoluene (TNT). The immunoreaction between a home-made polyclonal anti-2,4,6-trinitrophenyl-keyhole-limpet-hemocyanine antibody (P-TNPh-KLH Ab) with a physically immobilized TNPh-ovalbumin (TNPh-OVA) conjugate was utilized for TNT quantification based on the principle of indirect competitive immunoreaction. The performance of the P-TNPh-KLH Ab was compared with three commercially-available antibodies and was observed that the home-made antibody exhibited the highest sensitivity to TNT (1 ppt) among the other antibodies with a response time of 2 min.
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U2 - 10.5796/electrochemistry.74.141
DO - 10.5796/electrochemistry.74.141
M3 - Article
AN - SCOPUS:33645315818
SN - 1344-3542
VL - 74
SP - 141
EP - 144
JO - Electrochemistry
JF - Electrochemistry
IS - 2
ER -