Perfusion culture through a vasculature constructed in a tumor spheroid

Yuji Nashimoto, Yukako Teraoka, Akiko Nakamasu, Sanshiro Hanada, Yuichiro Arima, Yu Suke Torisawa, Hidetoshi Kotera, Koichi Nishiyama, Takashi Miura, Ryuji Yokokawa

Research output: Chapter in Book/Report/Conference proceedingConference contribution

Abstract

We developed a microfluidic system for a perfusion culture of tumor spheroids utilizing engineered vasculatures. By the definition of the cellular interaction between fibroblast cells in a tumor spheroid and endothelial cells in microchannels, we successfully induced angiogenic sprouts from the microchannels, which anastomosed with the spheroid and formed perfusable vasculatures. We continuously supplied medium to the tumor spheroid through the vasculature and evaluated the effect of perfusion on the growth of the tumor spheroid. Our perfusion system greatly improved the cellular proliferation activity, mimicking the nutrition supply and evacuation of waste product through vasculatures surrounding tumors in vivo.

Original languageEnglish
Title of host publication21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
PublisherChemical and Biological Microsystems Society
Pages1029-1030
Number of pages2
ISBN (Electronic)9780692941836
Publication statusPublished - Jan 1 2020
Event21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 - Savannah, United States
Duration: Oct 22 2017Oct 26 2017

Publication series

Name21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017

Conference

Conference21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017
CountryUnited States
CitySavannah
Period10/22/1710/26/17

All Science Journal Classification (ASJC) codes

  • Chemical Engineering (miscellaneous)
  • Bioengineering

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  • Cite this

    Nashimoto, Y., Teraoka, Y., Nakamasu, A., Hanada, S., Arima, Y., Torisawa, Y. S., Kotera, H., Nishiyama, K., Miura, T., & Yokokawa, R. (2020). Perfusion culture through a vasculature constructed in a tumor spheroid. In 21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017 (pp. 1029-1030). (21st International Conference on Miniaturized Systems for Chemistry and Life Sciences, MicroTAS 2017). Chemical and Biological Microsystems Society.