Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga cyanidioschyzon merolae

Takayuki Fujiwara, Osami Misumi, Kousuke Tashiro, Yamato Yoshida, Keiji Nishida, Fumi Yagisawa, Sousuke Imamura, Masaki Yoshida, Toshiyuki Mori, Kan Tanaka, Haruko Kuroiwa, Tsuneyoshi Kuroiwa

Research output: Contribution to journalArticle

54 Citations (Scopus)

Abstract

Previous cell cycle studies have been based on cell-nuclear proliferation only. Eukaryotic cells, however, have double membranes-bound organelles, such as the cell nucleus, mitochondrion, plastids and single-membrane-bound organelles such as ER, the Golgi body, vacuoles (lysosomes) and microbodies. Organelle proliferations, which are very important for cell functions, are poorly understood. To clarify this, we performed a microarray analysis during the cell cycle of Cyanidioschyzon merolae. C. merolae cells contain a minimum set of organelles that divide synchronously. The nuclear, mitochondrial and plastid genomes were completely sequenced. The results showed that, of 158 genes induced during the S or G2-M phase, 93 were known and contained genes related to mitochondrial division, ftsZ1-1, ftsz1-2 and mda1, and plastid division, ftsZ2-1, ftsZ2-2 and cmdnm2. Moreover, three genes, involved in vesicle trafficking between the single-membrane organelles such as vps29 and the Rab family protein, were identified and might be related to partitioning of single-membrane-bound organelles. In other genes, 46 were hypothetical and 19 were hypothetical conserved. The possibility of finding novel organelle division genes from hypothetical and hypothetical conserved genes in the S and G2-M expression groups is discussed.

Original languageEnglish
Pages (from-to)59-72
Number of pages14
JournalDNA Research
Volume16
Issue number1
DOIs
Publication statusPublished - Feb 1 2009

Fingerprint

Cell Nucleus Division
Rhodophyta
Organelles
Gene Expression
Genes
Plastids
Membranes
Cell Cycle
Plastid Genomes
Microbodies
Mitochondrial Genome
G2 Phase
Eukaryotic Cells
Microarray Analysis
Vacuoles
Lysosomes
Cell Nucleus
Cell Division
Mitochondria
Cell Proliferation

All Science Journal Classification (ASJC) codes

  • Molecular Biology
  • Genetics

Cite this

Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga cyanidioschyzon merolae. / Fujiwara, Takayuki; Misumi, Osami; Tashiro, Kousuke; Yoshida, Yamato; Nishida, Keiji; Yagisawa, Fumi; Imamura, Sousuke; Yoshida, Masaki; Mori, Toshiyuki; Tanaka, Kan; Kuroiwa, Haruko; Kuroiwa, Tsuneyoshi.

In: DNA Research, Vol. 16, No. 1, 01.02.2009, p. 59-72.

Research output: Contribution to journalArticle

Fujiwara, T, Misumi, O, Tashiro, K, Yoshida, Y, Nishida, K, Yagisawa, F, Imamura, S, Yoshida, M, Mori, T, Tanaka, K, Kuroiwa, H & Kuroiwa, T 2009, 'Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga cyanidioschyzon merolae', DNA Research, vol. 16, no. 1, pp. 59-72. https://doi.org/10.1093/dnares/dsn032
Fujiwara, Takayuki ; Misumi, Osami ; Tashiro, Kousuke ; Yoshida, Yamato ; Nishida, Keiji ; Yagisawa, Fumi ; Imamura, Sousuke ; Yoshida, Masaki ; Mori, Toshiyuki ; Tanaka, Kan ; Kuroiwa, Haruko ; Kuroiwa, Tsuneyoshi. / Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga cyanidioschyzon merolae. In: DNA Research. 2009 ; Vol. 16, No. 1. pp. 59-72.
@article{30258e3fb1d74cbd8a6a2ee2544938be,
title = "Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga cyanidioschyzon merolae",
abstract = "Previous cell cycle studies have been based on cell-nuclear proliferation only. Eukaryotic cells, however, have double membranes-bound organelles, such as the cell nucleus, mitochondrion, plastids and single-membrane-bound organelles such as ER, the Golgi body, vacuoles (lysosomes) and microbodies. Organelle proliferations, which are very important for cell functions, are poorly understood. To clarify this, we performed a microarray analysis during the cell cycle of Cyanidioschyzon merolae. C. merolae cells contain a minimum set of organelles that divide synchronously. The nuclear, mitochondrial and plastid genomes were completely sequenced. The results showed that, of 158 genes induced during the S or G2-M phase, 93 were known and contained genes related to mitochondrial division, ftsZ1-1, ftsz1-2 and mda1, and plastid division, ftsZ2-1, ftsZ2-2 and cmdnm2. Moreover, three genes, involved in vesicle trafficking between the single-membrane organelles such as vps29 and the Rab family protein, were identified and might be related to partitioning of single-membrane-bound organelles. In other genes, 46 were hypothetical and 19 were hypothetical conserved. The possibility of finding novel organelle division genes from hypothetical and hypothetical conserved genes in the S and G2-M expression groups is discussed.",
author = "Takayuki Fujiwara and Osami Misumi and Kousuke Tashiro and Yamato Yoshida and Keiji Nishida and Fumi Yagisawa and Sousuke Imamura and Masaki Yoshida and Toshiyuki Mori and Kan Tanaka and Haruko Kuroiwa and Tsuneyoshi Kuroiwa",
year = "2009",
month = "2",
day = "1",
doi = "10.1093/dnares/dsn032",
language = "English",
volume = "16",
pages = "59--72",
journal = "DNA Research",
issn = "1340-2838",
publisher = "Oxford University Press",
number = "1",

}

TY - JOUR

T1 - Periodic gene expression patterns during the highly synchronized cell nucleus and organelle division cycles in the unicellular red alga cyanidioschyzon merolae

AU - Fujiwara, Takayuki

AU - Misumi, Osami

AU - Tashiro, Kousuke

AU - Yoshida, Yamato

AU - Nishida, Keiji

AU - Yagisawa, Fumi

AU - Imamura, Sousuke

AU - Yoshida, Masaki

AU - Mori, Toshiyuki

AU - Tanaka, Kan

AU - Kuroiwa, Haruko

AU - Kuroiwa, Tsuneyoshi

PY - 2009/2/1

Y1 - 2009/2/1

N2 - Previous cell cycle studies have been based on cell-nuclear proliferation only. Eukaryotic cells, however, have double membranes-bound organelles, such as the cell nucleus, mitochondrion, plastids and single-membrane-bound organelles such as ER, the Golgi body, vacuoles (lysosomes) and microbodies. Organelle proliferations, which are very important for cell functions, are poorly understood. To clarify this, we performed a microarray analysis during the cell cycle of Cyanidioschyzon merolae. C. merolae cells contain a minimum set of organelles that divide synchronously. The nuclear, mitochondrial and plastid genomes were completely sequenced. The results showed that, of 158 genes induced during the S or G2-M phase, 93 were known and contained genes related to mitochondrial division, ftsZ1-1, ftsz1-2 and mda1, and plastid division, ftsZ2-1, ftsZ2-2 and cmdnm2. Moreover, three genes, involved in vesicle trafficking between the single-membrane organelles such as vps29 and the Rab family protein, were identified and might be related to partitioning of single-membrane-bound organelles. In other genes, 46 were hypothetical and 19 were hypothetical conserved. The possibility of finding novel organelle division genes from hypothetical and hypothetical conserved genes in the S and G2-M expression groups is discussed.

AB - Previous cell cycle studies have been based on cell-nuclear proliferation only. Eukaryotic cells, however, have double membranes-bound organelles, such as the cell nucleus, mitochondrion, plastids and single-membrane-bound organelles such as ER, the Golgi body, vacuoles (lysosomes) and microbodies. Organelle proliferations, which are very important for cell functions, are poorly understood. To clarify this, we performed a microarray analysis during the cell cycle of Cyanidioschyzon merolae. C. merolae cells contain a minimum set of organelles that divide synchronously. The nuclear, mitochondrial and plastid genomes were completely sequenced. The results showed that, of 158 genes induced during the S or G2-M phase, 93 were known and contained genes related to mitochondrial division, ftsZ1-1, ftsz1-2 and mda1, and plastid division, ftsZ2-1, ftsZ2-2 and cmdnm2. Moreover, three genes, involved in vesicle trafficking between the single-membrane organelles such as vps29 and the Rab family protein, were identified and might be related to partitioning of single-membrane-bound organelles. In other genes, 46 were hypothetical and 19 were hypothetical conserved. The possibility of finding novel organelle division genes from hypothetical and hypothetical conserved genes in the S and G2-M expression groups is discussed.

UR - http://www.scopus.com/inward/record.url?scp=65249090864&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=65249090864&partnerID=8YFLogxK

U2 - 10.1093/dnares/dsn032

DO - 10.1093/dnares/dsn032

M3 - Article

C2 - 19147531

AN - SCOPUS:65249090864

VL - 16

SP - 59

EP - 72

JO - DNA Research

JF - DNA Research

SN - 1340-2838

IS - 1

ER -