Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function

The factor is osteoprotegerin/ osteoclastogenesis inhibitory factor

Naohisa Wada, Hidefumi Maeda, Kazunari Tanabe, Eisuke Tsuda, Kazuki Yano, Hiroyoshi Nakamuta, Akifumi Akamine

Research output: Contribution to journalArticle

76 Citations (Scopus)

Abstract

The periodontal ligament, a highly specialized connective tissue situated between the tooth and the alveolar bone of the tooth socket, has been thought to influence the remodeling of the alveolar bone. The effects of two human periodontal ligament fibroblastic cell populations (HPLFs) on osteoclast-like cell (OCL) formation and the function of authentic osteoclasts were examined. The addition of the conditioned media (CM) from both HPLF cultures (HPLF-CMs) to mouse bone marrow culture inhibited OCL formation in spite of the presence of 10-8 M 1α, 25 dihydroxyvitamin D3 (1α,25(OH)2D3). This inhibitory effect was most remarkable when both CMs were added during day 6 to day 9 following bone marrow culture, just at the late stage of OCL differentiation. HPLF-CMs also induced a significant decrease in the pit area and the pit number formed by authentic osteoclasts on ivory slices. The administration of neutralizing monoclonal antibody (OI-1 ) against human osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) with HPLF-CMs to mouse bone marrow culture almost completely blocked the inhibitory effect of these CMs on OCL formation. Immunofluorescent examination of HPLF with OI-1 revealed intense positive reactivity in the cytoplasm. Western blot analysis of HPLF-CM using anti-human OPG/OCIF polyclonal antibody resulted in the detection of bands of 60 kDa and 120 kDa which were consistent with those of OPG/OCIF. These results suggest that HPLF cells produce and secrete OPG/OCIF, and that this factor from HPLF prevents the differentiation of the late preosteoclast and the function of the mature osteoclasts.

Original languageEnglish
Pages (from-to)56-63
Number of pages8
JournalJournal of Periodontal Research
Volume36
Issue number1
DOIs
Publication statusPublished - Jan 1 2001

Fingerprint

Osteoprotegerin
Periodontal Ligament
Osteoclasts
Bone Marrow
Conditioned Culture Medium
Tooth Socket
Calcitriol
Bone Remodeling
Neutralizing Antibodies
Connective Tissue
Cell Differentiation
Tooth
Cytoplasm
Western Blotting
Monoclonal Antibodies
Bone and Bones
Antibodies

All Science Journal Classification (ASJC) codes

  • Periodontics

Cite this

Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function : The factor is osteoprotegerin/ osteoclastogenesis inhibitory factor. / Wada, Naohisa; Maeda, Hidefumi; Tanabe, Kazunari; Tsuda, Eisuke; Yano, Kazuki; Nakamuta, Hiroyoshi; Akamine, Akifumi.

In: Journal of Periodontal Research, Vol. 36, No. 1, 01.01.2001, p. 56-63.

Research output: Contribution to journalArticle

@article{bd1de7920de343bbab771cbf81ccce15,
title = "Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function: The factor is osteoprotegerin/ osteoclastogenesis inhibitory factor",
abstract = "The periodontal ligament, a highly specialized connective tissue situated between the tooth and the alveolar bone of the tooth socket, has been thought to influence the remodeling of the alveolar bone. The effects of two human periodontal ligament fibroblastic cell populations (HPLFs) on osteoclast-like cell (OCL) formation and the function of authentic osteoclasts were examined. The addition of the conditioned media (CM) from both HPLF cultures (HPLF-CMs) to mouse bone marrow culture inhibited OCL formation in spite of the presence of 10-8 M 1α, 25 dihydroxyvitamin D3 (1α,25(OH)2D3). This inhibitory effect was most remarkable when both CMs were added during day 6 to day 9 following bone marrow culture, just at the late stage of OCL differentiation. HPLF-CMs also induced a significant decrease in the pit area and the pit number formed by authentic osteoclasts on ivory slices. The administration of neutralizing monoclonal antibody (OI-1 ) against human osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) with HPLF-CMs to mouse bone marrow culture almost completely blocked the inhibitory effect of these CMs on OCL formation. Immunofluorescent examination of HPLF with OI-1 revealed intense positive reactivity in the cytoplasm. Western blot analysis of HPLF-CM using anti-human OPG/OCIF polyclonal antibody resulted in the detection of bands of 60 kDa and 120 kDa which were consistent with those of OPG/OCIF. These results suggest that HPLF cells produce and secrete OPG/OCIF, and that this factor from HPLF prevents the differentiation of the late preosteoclast and the function of the mature osteoclasts.",
author = "Naohisa Wada and Hidefumi Maeda and Kazunari Tanabe and Eisuke Tsuda and Kazuki Yano and Hiroyoshi Nakamuta and Akifumi Akamine",
year = "2001",
month = "1",
day = "1",
doi = "10.1034/j.1600-0765.2001.00604.x",
language = "English",
volume = "36",
pages = "56--63",
journal = "Journal of Periodontal Research",
issn = "0022-3484",
publisher = "Blackwell Munksgaard",
number = "1",

}

TY - JOUR

T1 - Periodontal ligament cells secrete the factor that inhibits osteoclastic differentiation and function

T2 - The factor is osteoprotegerin/ osteoclastogenesis inhibitory factor

AU - Wada, Naohisa

AU - Maeda, Hidefumi

AU - Tanabe, Kazunari

AU - Tsuda, Eisuke

AU - Yano, Kazuki

AU - Nakamuta, Hiroyoshi

AU - Akamine, Akifumi

PY - 2001/1/1

Y1 - 2001/1/1

N2 - The periodontal ligament, a highly specialized connective tissue situated between the tooth and the alveolar bone of the tooth socket, has been thought to influence the remodeling of the alveolar bone. The effects of two human periodontal ligament fibroblastic cell populations (HPLFs) on osteoclast-like cell (OCL) formation and the function of authentic osteoclasts were examined. The addition of the conditioned media (CM) from both HPLF cultures (HPLF-CMs) to mouse bone marrow culture inhibited OCL formation in spite of the presence of 10-8 M 1α, 25 dihydroxyvitamin D3 (1α,25(OH)2D3). This inhibitory effect was most remarkable when both CMs were added during day 6 to day 9 following bone marrow culture, just at the late stage of OCL differentiation. HPLF-CMs also induced a significant decrease in the pit area and the pit number formed by authentic osteoclasts on ivory slices. The administration of neutralizing monoclonal antibody (OI-1 ) against human osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) with HPLF-CMs to mouse bone marrow culture almost completely blocked the inhibitory effect of these CMs on OCL formation. Immunofluorescent examination of HPLF with OI-1 revealed intense positive reactivity in the cytoplasm. Western blot analysis of HPLF-CM using anti-human OPG/OCIF polyclonal antibody resulted in the detection of bands of 60 kDa and 120 kDa which were consistent with those of OPG/OCIF. These results suggest that HPLF cells produce and secrete OPG/OCIF, and that this factor from HPLF prevents the differentiation of the late preosteoclast and the function of the mature osteoclasts.

AB - The periodontal ligament, a highly specialized connective tissue situated between the tooth and the alveolar bone of the tooth socket, has been thought to influence the remodeling of the alveolar bone. The effects of two human periodontal ligament fibroblastic cell populations (HPLFs) on osteoclast-like cell (OCL) formation and the function of authentic osteoclasts were examined. The addition of the conditioned media (CM) from both HPLF cultures (HPLF-CMs) to mouse bone marrow culture inhibited OCL formation in spite of the presence of 10-8 M 1α, 25 dihydroxyvitamin D3 (1α,25(OH)2D3). This inhibitory effect was most remarkable when both CMs were added during day 6 to day 9 following bone marrow culture, just at the late stage of OCL differentiation. HPLF-CMs also induced a significant decrease in the pit area and the pit number formed by authentic osteoclasts on ivory slices. The administration of neutralizing monoclonal antibody (OI-1 ) against human osteoprotegerin (OPG)/osteoclastogenesis inhibitory factor (OCIF) with HPLF-CMs to mouse bone marrow culture almost completely blocked the inhibitory effect of these CMs on OCL formation. Immunofluorescent examination of HPLF with OI-1 revealed intense positive reactivity in the cytoplasm. Western blot analysis of HPLF-CM using anti-human OPG/OCIF polyclonal antibody resulted in the detection of bands of 60 kDa and 120 kDa which were consistent with those of OPG/OCIF. These results suggest that HPLF cells produce and secrete OPG/OCIF, and that this factor from HPLF prevents the differentiation of the late preosteoclast and the function of the mature osteoclasts.

UR - http://www.scopus.com/inward/record.url?scp=0035259647&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0035259647&partnerID=8YFLogxK

U2 - 10.1034/j.1600-0765.2001.00604.x

DO - 10.1034/j.1600-0765.2001.00604.x

M3 - Article

VL - 36

SP - 56

EP - 63

JO - Journal of Periodontal Research

JF - Journal of Periodontal Research

SN - 0022-3484

IS - 1

ER -