TY - JOUR
T1 - Periostin promotes the generation of fibrous membranes in proliferative vitreoretinopathy Keijiro Ishikawa1
AU - Yoshida, Shigeo
AU - Nakao, Shintaro
AU - Nakama, Takahito
AU - Kita, Takeshi
AU - Asato, Ryo
AU - Sassa, Yukio
AU - Arita, Ryoichi
AU - Miyazaki, Masanori
AU - Enaida, Hiroshi
AU - Oshima, Yuji
AU - Murakami, Noboru
AU - Niiro, Hiroaki
AU - Ono, Junya
AU - Matsuda, Akira
AU - Goto, Yoshinobu
AU - Akashi, Koichi
AU - Izuhara, Kenji
AU - Kudo, Akira
AU - Kono, Toshihiro
AU - Hafezi-Moghadam, Ali
AU - Ishibashi, Tatsuro
PY - 2014/1
Y1 - 2014/1
N2 - Proliferative vitreoretinopathy (PVR) is a severe, vision-threatening disorder characterized by the fibrous membrane formation that leads to tractional retinal detachment. There has been no effective therapeutic approach other than vitreoretinal surgery. In this study, DNA microarray analysis of the fibrous membranes revealed significant up-regulation of periostin. We also found increased periostin expression in the vitreous and retinal pigment epithelial (RPE) cells from fibrous membranes of PVR patients. In vitro, periostin increased proliferation, adhesion, migration, and collagen production in RPE cells through integrin αV-mediated FAK and AKT phosphorylation. Periostin blockade suppressed migration and adhesion induced by TGFα2 and PVR vitreous. In vivo, periostin inhibition had the inhibitory effect on progression of experimental PVR in rabbit eyes without affecting the viability of retinal cells. These results identified periostin as a pivotal molecule for fibrous membrane formation as well as a promising therapeutic target for PVR.
AB - Proliferative vitreoretinopathy (PVR) is a severe, vision-threatening disorder characterized by the fibrous membrane formation that leads to tractional retinal detachment. There has been no effective therapeutic approach other than vitreoretinal surgery. In this study, DNA microarray analysis of the fibrous membranes revealed significant up-regulation of periostin. We also found increased periostin expression in the vitreous and retinal pigment epithelial (RPE) cells from fibrous membranes of PVR patients. In vitro, periostin increased proliferation, adhesion, migration, and collagen production in RPE cells through integrin αV-mediated FAK and AKT phosphorylation. Periostin blockade suppressed migration and adhesion induced by TGFα2 and PVR vitreous. In vivo, periostin inhibition had the inhibitory effect on progression of experimental PVR in rabbit eyes without affecting the viability of retinal cells. These results identified periostin as a pivotal molecule for fibrous membrane formation as well as a promising therapeutic target for PVR.
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U2 - 10.1096/fj.13-229740
DO - 10.1096/fj.13-229740
M3 - Article
C2 - 24022401
AN - SCOPUS:84894562099
SN - 0892-6638
VL - 28
SP - 131
EP - 142
JO - FASEB Journal
JF - FASEB Journal
IS - 1
ER -