Periostin promotes the generation of fibrous membranes in proliferative vitreoretinopathy Keijiro Ishikawa1

Shigeo Yoshida, Shintaro Nakao, Takahito Nakama, Takeshi Kita, Ryo Asato, Yukio Sassa, Ryoichi Arita, Masanori Miyazaki, Hiroshi Enaida, Yuji Oshima, Noboru Murakami, Hiroaki Niiro, Junya Ono, Akira Matsuda, Yoshinobu Goto, Koichi Akashi, Kenji Izuhara, Akira Kudo, Toshihiro Kono, Ali Hafezi-MoghadamTatsuro Ishibashi

Research output: Contribution to journalArticle

43 Citations (Scopus)

Abstract

Proliferative vitreoretinopathy (PVR) is a severe, vision-threatening disorder characterized by the fibrous membrane formation that leads to tractional retinal detachment. There has been no effective therapeutic approach other than vitreoretinal surgery. In this study, DNA microarray analysis of the fibrous membranes revealed significant up-regulation of periostin. We also found increased periostin expression in the vitreous and retinal pigment epithelial (RPE) cells from fibrous membranes of PVR patients. In vitro, periostin increased proliferation, adhesion, migration, and collagen production in RPE cells through integrin αV-mediated FAK and AKT phosphorylation. Periostin blockade suppressed migration and adhesion induced by TGFα2 and PVR vitreous. In vivo, periostin inhibition had the inhibitory effect on progression of experimental PVR in rabbit eyes without affecting the viability of retinal cells. These results identified periostin as a pivotal molecule for fibrous membrane formation as well as a promising therapeutic target for PVR.

Original languageEnglish
Pages (from-to)131-142
Number of pages12
JournalFASEB Journal
Volume28
Issue number1
DOIs
Publication statusPublished - Jan 2014

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All Science Journal Classification (ASJC) codes

  • Biotechnology
  • Biochemistry
  • Molecular Biology
  • Genetics

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