Peripheral administration of kiss1 pentadecapeptide induces gonadal development in sexually immature adult scombroid fish

Sethu Selvaraj, Hirofumi Ohga, Hajime Kitano, Mitsuo Nyuji, Akihiko Yamaguchi, Michiya Matsuyama

Research output: Contribution to journalArticle

25 Citations (Scopus)

Abstract

Kisspeptins have emerged as potent regulators of the reproductive brain-pituitary-gonad (BPG) axis. Our previous study demonstrated that the brain of the chub mackerel (Scomber japonicus), a scombroid fish, expresses two kisspeptin-encoding genes, kiss1 and kiss2, and exhibits sexually dimorphic expression profiles. Recent studies strongly suggest that teleost Kiss1 and Kiss2 precursors produce mature Kiss1-pentadecapeptides (Kiss1-15) and Kiss2-dodecapeptides (Kiss2-12), respectively. In light of the above, the present study evaluated the potency of synthetic peptides of Kiss1-15, Kiss2-12, and a GnRH analog (GnRHa) on inducing gonadal development in sexually immature adult chub mackerel. Synthetic peptides were administered subcutaneously through mini-osmotic pumps. On day 45 post-administration, gonadosomatic index (GSI) values (%) of male fish treated with Kiss1-15 (1.82) significantly increased in comparison to initial control (0.33), final control (0.49), Kiss2-12 (0.24), and GnRHa (1.13)-treated fish. Interestingly, the testis of all Kiss1-15 treated fish revealed spermiation, and were full of spermatozoa. These fish showed significantly higher levels of pituitary fsh and Ih mRNAs and circulating 11-ketotestosterone. GnRHa treated fish also revealed the presence of few spermatozoa in the testis. In females, no significant changes in GSI values were found between treatments; however, Kiss1-15- and GnRHa-treated fish showed prominent signs of vitellogenic onset, with many early yolk oocytes in their ovaries. Interestingly, Kiss1-15-treated fish exhibited higher levels of pituitary fsh and circulating estradiol-17. These results indicate that peripheral administration of Kiss1-15 and GnRHa can induce gonadal development in sexually immature chub mackerel.

Original languageEnglish
Pages (from-to)446-454
Number of pages9
JournalZoological Science
Volume30
Issue number6
DOIs
Publication statusPublished - Jun 2013

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immatures
Scomber japonicus
fish
synthetic peptides
gonadosomatic index
testes
spermatozoa
brain
spermiation
pumps
estradiol
gonads
oocytes
genes

All Science Journal Classification (ASJC) codes

  • Animal Science and Zoology

Cite this

Peripheral administration of kiss1 pentadecapeptide induces gonadal development in sexually immature adult scombroid fish. / Selvaraj, Sethu; Ohga, Hirofumi; Kitano, Hajime; Nyuji, Mitsuo; Yamaguchi, Akihiko; Matsuyama, Michiya.

In: Zoological Science, Vol. 30, No. 6, 06.2013, p. 446-454.

Research output: Contribution to journalArticle

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abstract = "Kisspeptins have emerged as potent regulators of the reproductive brain-pituitary-gonad (BPG) axis. Our previous study demonstrated that the brain of the chub mackerel (Scomber japonicus), a scombroid fish, expresses two kisspeptin-encoding genes, kiss1 and kiss2, and exhibits sexually dimorphic expression profiles. Recent studies strongly suggest that teleost Kiss1 and Kiss2 precursors produce mature Kiss1-pentadecapeptides (Kiss1-15) and Kiss2-dodecapeptides (Kiss2-12), respectively. In light of the above, the present study evaluated the potency of synthetic peptides of Kiss1-15, Kiss2-12, and a GnRH analog (GnRHa) on inducing gonadal development in sexually immature adult chub mackerel. Synthetic peptides were administered subcutaneously through mini-osmotic pumps. On day 45 post-administration, gonadosomatic index (GSI) values ({\%}) of male fish treated with Kiss1-15 (1.82) significantly increased in comparison to initial control (0.33), final control (0.49), Kiss2-12 (0.24), and GnRHa (1.13)-treated fish. Interestingly, the testis of all Kiss1-15 treated fish revealed spermiation, and were full of spermatozoa. These fish showed significantly higher levels of pituitary fsh and Ih mRNAs and circulating 11-ketotestosterone. GnRHa treated fish also revealed the presence of few spermatozoa in the testis. In females, no significant changes in GSI values were found between treatments; however, Kiss1-15- and GnRHa-treated fish showed prominent signs of vitellogenic onset, with many early yolk oocytes in their ovaries. Interestingly, Kiss1-15-treated fish exhibited higher levels of pituitary fsh and circulating estradiol-17. These results indicate that peripheral administration of Kiss1-15 and GnRHa can induce gonadal development in sexually immature chub mackerel.",
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